論文

査読有り
2014年8月17日

“Mechano-sensing” by actin filaments

Transactions of Japanese Society for Medical and Biological Engineering
  • Kimihide Hayakawa
  • ,
  • Masahiro Sokabe
  • ,
  • Hitoshi Tatsumi

52
開始ページ
46
終了ページ
SY-47
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.11239/jsmbe.52.SY-46
出版者・発行元
Japan Soc. of Med. Electronics and Biol. Engineering

Mechanical forces play crucial roles in a variety of cell functions, including growth, survival, proliferation, and migration. Rearrangement of actin cytoskeletal structures is one of the typical cell events in response to mechanical forces. For example, formation of actin stress fibers is greatly affected by tension increases in themselves induced by intrinsic contractile forces or extrinsic stretching forces. Here, we propose a novel hypothesis, “mechano-sensingby actin filaments”. To test this hypothesis, we examined the effect of actin-modulating protein cofilin to single actin filaments with or without tension in the filaments. Relaxed actin filaments were severed by cofilin in less than ca. 20 s, whereas they were severed with a larger delay when the filaments were tensed. Single molecular imaging of cofilin bindings showed that the rate of cofilin binding to an actin filament was decreased in tensed filaments in comparison with relaxed filaments. These observations suggest that tension in the filament affects the apparent severing activity of cofilin by decreasing the on-rate of cofilin binding, and support the hypothesis that actin filaments act as a “negative tension senor”.

リンク情報
DOI
https://doi.org/10.11239/jsmbe.52.SY-46
ID情報
  • DOI : 10.11239/jsmbe.52.SY-46
  • ISSN : 1347-443X
  • ISSN : 1881-4379
  • SCOPUS ID : 84939450920

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