Electron-spin resonance (ESR) for the charge-ordered state in well-characterized Pr0.65Ca0.35MnO3 exhibited significant change of both the absorption profile and the effective spin susceptibility upon injection of laser light with photon energy of 1.17eV provided by Nd-YAG laser. The increase of the effective spin susceptibility was clearly found out thanks to the injection of photons in the temperature range 90 K-80 K, which is below the transition temperature from the antiferromagnetic charge ordered state to the canted antiferromagnetic spin alternation state, TCAF∼125K. The temperature dependence of the change of the ESR profile excludes the possibility of heating by laser light. The present result suggests that a kind of photo-induced insulator-metal transition may occur due to propagation of the delocalized carriers via probable double exchange interaction in the charge-ordering collapsed state created by the injection of photons. © 1999 Plenum Publishing Corporation.