論文

査読有り
2006年10月

A hepatocyte growth factor (HGF)/receptor autocrine loop regulates constitutive self-renewal of human periodontal ligament cells but reduces sensitivity to exogenous HGF

JOURNAL OF PERIODONTOLOGY
  • Tomoyuki Kawase
  • ,
  • Kazuhiro Okuda
  • ,
  • Hiromasa Yoshie

77
10
開始ページ
1723
終了ページ
1730
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1902/jop.2006.060031
出版者・発行元
AMER ACAD PERIODONTOLOGY

Background: In addition to its prominent role in liver regeneration, hepatocyte growth factor (HGF) is now generally thought to be produced by mesenchymal cells to promote the regeneration of epithelial tissue by a paracrine mechanism. However, it is not known how or if HGF could be involved in the regeneration of periodontal tissues. The purpose of this study was to characterize the ability of normal human periodontal ligament (PDL) cells to produce or respond to HGF.
Methods: PDL cells derived from healthy young volunteers were used from passages four through 10. HGF receptors were detected both by immunocytochemical staining and Western-blotting analysis. Both DNA synthesis (by bromo-deoxyuridine [BrdU]-incorporation) and secreted HGF were quantified by enzyme-linked immunosorbent assays. Mitogen -activated protein kinase (MAPK) phosphorylation was also analyzed by Western blot.
Results: Despite the immunocytochemical demonstration of HGF receptor protein in the cytoplasm and on the plasma membrane of PDL cells, exogenous recombinant human HGF did not exert the mitogenic effects expected. As reported for other mesenchymal cells, PDL cells were found to secrete HGF. Treatments with neutralizing anti-HGF antibody significantly suppressed constitutive PDL cell proliferation and sustained the receptor protein at higher levels than in non-treated cells. Under these conditions, exogenous HGF rapidly phosphorylated extracellular signal-regulated kinase (ERK), an action linked to the cell proliferation and downregulation of cell-surface receptors.
Conclusions: Unlike other known mesenchymal or epithelial cells, these findings suggest that normal PDL cells from young donors possess a constitutive HGF/receptor autocrine loop that normally regulates their replacement self-proliferation but reduces sensitivity to exogenously applied HGF by acute receptor downregulation.

リンク情報
DOI
https://doi.org/10.1902/jop.2006.060031
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000241879700015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1902/jop.2006.060031
  • ISSN : 0022-3492
  • Web of Science ID : WOS:000241879700015

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