2008年1月
Altered gene expression levels of matrix metalloproteinases and their inhibitors in periodontitis-affected gingival tissue
Journal of Periodontology
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- 巻
- 79
- 号
- 1
- 開始ページ
- 166
- 終了ページ
- 173
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1902/jop.2008.070159
- 出版者・発行元
- AMER ACAD PERIODONTOLOGY
Background: The balance between the degradation and synthesis of extracellular matrix determines periodontal attachment levels and alveolar bone matrix concentration in periodontal diseases. Matrix metalloproteinases (MMPs) are known to degrade periodontal ligamental attachment and bone matrix proteins. The purpose of this study was to examine the effect of different expression levels of MMPs and their inhibitors, the tissue inhibitors of matrix metalloproteinases (TIMPs), in periodontitis.
Methods: Sixteen inflamed gingival tissue samples from subjects with generalized chronic periodontitis and 14 control tissue samples from systemically and periodontally healthy subjects were evaluated. The total RNA was extracted, and the transcript levels for MMP-1, -3, -9, and -13 and TIMP-1, -2, -3, and -4 relative to P-actin were determined by quantitative real-time reverse transcription - polymerase chain reaction.
Results: Gene transcript levels for MMP-1 and TIMP-4 were significantly higher in periodontitis-affected gingival tissues (P <0.05). MMP-3, -9, and - 13 and TIMP-1 mRNAs also were elevated in periodontitis; however, the difference was not statistically significant. TIMP-2 and -3 mRNA levels were similar in healthy and diseased gingivae. The ratios of MMP-1 /TIMP-2 (P <0.01), MMP-3/TlMP-2 (P<0.05), MMP-9/TIMP-2 (P<0.05), and MMP-1/TIMP-3 (P<0.01) from periodontitis lesions were significantly higher than those in the control tissues.
Conclusions: Upregulated MMP expression and an increased MMP/TIMP ratio indicate that a potential imbalance between degradation and synthesis of extracellular matrix persists in periodontitis-affected gingival tissues. This process may be responsible for increased tissue breakdown in periodontitis.
Methods: Sixteen inflamed gingival tissue samples from subjects with generalized chronic periodontitis and 14 control tissue samples from systemically and periodontally healthy subjects were evaluated. The total RNA was extracted, and the transcript levels for MMP-1, -3, -9, and -13 and TIMP-1, -2, -3, and -4 relative to P-actin were determined by quantitative real-time reverse transcription - polymerase chain reaction.
Results: Gene transcript levels for MMP-1 and TIMP-4 were significantly higher in periodontitis-affected gingival tissues (P <0.05). MMP-3, -9, and - 13 and TIMP-1 mRNAs also were elevated in periodontitis; however, the difference was not statistically significant. TIMP-2 and -3 mRNA levels were similar in healthy and diseased gingivae. The ratios of MMP-1 /TIMP-2 (P <0.01), MMP-3/TlMP-2 (P<0.05), MMP-9/TIMP-2 (P<0.05), and MMP-1/TIMP-3 (P<0.01) from periodontitis lesions were significantly higher than those in the control tissues.
Conclusions: Upregulated MMP expression and an increased MMP/TIMP ratio indicate that a potential imbalance between degradation and synthesis of extracellular matrix persists in periodontitis-affected gingival tissues. This process may be responsible for increased tissue breakdown in periodontitis.
- リンク情報
- ID情報
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- DOI : 10.1902/jop.2008.070159
- ISSN : 0022-3492
- eISSN : 1943-3670
- PubMed ID : 18166107
- Web of Science ID : WOS:000252256100022