2008年2月
The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice
BIOMEDICAL RESEARCH-TOKYO
- ,
- ,
- 巻
- 29
- 号
- 1
- 開始ページ
- 33
- 終了ページ
- 42
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2220/biomedres.29.33
- 出版者・発行元
- BIOMEDICAL RESEARCH PRESS LTD
The calcitonin-gene related peptide (CGRP) is a primary afferent neurotransmitter in the trigeminal system. Although a neonatal administration of capsaicin eliminates substance P (SP)-mediated nociceptive responses to induce a permanent functional reduction in C-fibers, little information is available regarding changes in CGRP-immunoreaction in mice undergoing neonatal capsaicin treatment (CP mice). This study examined postnatal changes in the distribution of CGRP-immunoreaction in the trigeminal subnucleus caudalis and trigeminal ganglion of CP mice by immunohistochemical technique and a quantitative analysis. Immunohistochemistry for CGRP in the subnucleus caudalis (Vc) demonstrated two dense distributions of neurons in the CP mice as well as naive mice: in the marginal layer and the region 400-600 mu m deep. The quantitative analysis revealed no significant difference in the density of CGRP immunoreaction between naive and CP mice 1-8 weeks of age. In the trigeminal ganglion of both groups, the size distribution of CGRP-positive neurons displayed a distribution pattern with one peak in 200-300 mu m(2) at week 1 and with two peaks in 200-300 mu m(2) and 600-700 mu m(2) at week 8 but no significant difference in neural density existed between these regions. When double staining in the naive mice with CGRP or SP and VR1, a capsaicin receptor, was done, many trigeminal ganglion neurons co-expressed SP- and VR1-immunoreactions, but rarely exhibited CGRP/VR1-co-localization. Taken together with previous data, these current observations suggest that CGRP containing afferent neurons possibly performs differing roles in nociceptive afferent input transmission within the Vc from SP-containing neurons in mice.
- リンク情報
- ID情報
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- DOI : 10.2220/biomedres.29.33
- ISSN : 0388-6107
- PubMed ID : 18344596
- Web of Science ID : WOS:000253926600005