論文

査読有り 責任著者
2005年12月

Postnatal development of substance P-immunoreaction in the trigerninal caudalis of neonatally capsaicin-treated mice

ARCHIVES OF HISTOLOGY AND CYTOLOGY
  • M Aita
  • ,
  • T Maeda
  • ,
  • R Takagi
  • ,
  • K Seo

68
4
開始ページ
311
終了ページ
320
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1679/aohc.68.311
出版者・発行元
INT SOC HISTOLOGY & CYTOLOGY

The trigeminal subnucleus caudalis (Vc) is a critical relay site for processing nociceptive afferent input from the orofacial area in addition to its modulation by neuroplastic change. Although an administration of capsaicin in neonates induces a selective destruction of substance P (SP)-immunoreactive nerve fibers, little information is available regarding its detailed effects on the Vc, particularly during postnatal development. The present study examined postnatal changes in the distribution of SP in the Vc and trigeminal ganglion (TG) by immunohistochemical techniques in naive (NV) and neonatally capsaicin-treated (CP) mice, combined with a quantitative analysis. The neonatal mice received a single subcutaneous injection of capsaicin (50 mg/kg) at 48 hours after birth. The neural density of the SP-immunoreaction decreased to approximately a quarter of that in 1-week-old NV mice but increased to three-quarters of that in the NV in the superficial area after postnatal week 2. A double staining with SP and myelin basic protein confirmed the absence of any SP-immunoreaction in the myelinated nerve fibers in both NV and CP mice. The SP-immunoreaction never overlapped with non-peptidergic IB4-labeled neurons in the Vc and TG of either group. Neither the size distribution of SP-positive neurons nor their relative ratio in the TG differed between NV and CP mice at the ages of postnatal weeks 1 and 8. These findings indicate two putative origins for the emergent SP-immunoreaction in the superficial layer of the Vc of the CP mice: the surviving trigeminal neurons with SP against capsaicin treatment and/or intrinsic neurons/interneurons in the Vc without SP under normal conditions.

リンク情報
DOI
https://doi.org/10.1679/aohc.68.311
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16477150
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000235066200010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1679/aohc.68.311
  • ISSN : 0914-9465
  • eISSN : 1349-1717
  • PubMed ID : 16477150
  • Web of Science ID : WOS:000235066200010

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