The basic subunit of glutelin from rice dry seeds were shown to have N-linked sugar chains by lectin staining with PHA-E4, WGA and Con A. NaIO4 and N-glycanase treatments also provided the evidences of N-linked sugar chains. The purified basic subunit was applied to NEPHGE and eight isoforms of it were separated. The fractionated eight isoforms were shown to be nearly homogeneous by C18-silica column chromatography. Each was subject to lectin staining. The eight isoforms were hybridized with PNA, PHA-E4 and Con A. This lectin staining indicated that each of the isoforms of the basic subunit had N-linked sugar chain. This is the first report showing the occurrence of N-linked sugar chain in isoforms of glutelin basic subunit.