2007
Proteomic characterization of tissue expansion of rice scutellum stimulated by abscisic acid
Bioscience, Biotechnology and Biochemistry
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- Volume
- 71
- Number
- 5
- First page
- 1260
- Last page
- 1268
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1271/bbb.60675
We found that appropriate treatment with a highly potent and long-lasting abscisic acid analog enhanced the tissue expansion of scutellum during early seedling development of rice, accompanied by increases of protein and starch accumulation in the tissue. A comparative display of the protein expression patterns in the abscisic acid analog-treated and non-treated tissues on two dimensional gel electrophoretogram indicated that approximately 30% of the scutellar proteins were induced by abscisic acid. The abscisic acid-induced proteins included sucrose metabolizing, glycolytic, and ATP-producing enzymes. Most of these enzyme proteins also increased during the seedling growth. In addition, the expression of some isoforms of UDP-glucose pyrophosphorylase, 3-phosphoglycerate kinase, and mitochondrial ATP synthase beta chain was stimulated in the scutellum, with suppressed expression of α-amylase. We concluded that abscisic acid directly and indirectly stimulates the expression of numerous proteins, including carbohydrate metabolic enzymes, in scutellar tissues.
- Link information
- ID information
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- DOI : 10.1271/bbb.60675
- ISSN : 0916-8451
- ISSN : 1347-6947
- Pubmed ID : 17485861
- SCOPUS ID : 34347369371