Dec, 2015
Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice
PLANT BIOTECHNOLOGY JOURNAL
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- Volume
- 13
- Number
- 9
- First page
- 1251
- Last page
- 1263
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1111/pbi.12314
- Publisher
- WILEY-BLACKWELL
Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat-stress-tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence contains a signal sequence and an N-glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD1-YFP showed MSD1-YFP in the Golgi apparatus and plastids, indicating that MSD1 is a unique Golgi/plastid-type SOD. To evaluate the involvement of MSD1 in heat-stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD1. The grain quality of rice with constitutive high expression of MSD1 grown at 33/28 degrees C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD1-knock-down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD1 up-regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD1 plays an important role in adaptation to heat stress.
- Link information
- ID information
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- DOI : 10.1111/pbi.12314
- ISSN : 1467-7644
- eISSN : 1467-7652
- Web of Science ID : WOS:000368287600005