MISC

2003年5月

Expression and functional characterization of the adhesion molecule spermatogenic immunoglobulin superfamily in the mouse testis

BIOLOGY OF REPRODUCTION
  • T Wakayama
  • ,
  • H Koami
  • ,
  • H Ariga
  • ,
  • D Kobayashi
  • ,
  • Y Sai
  • ,
  • A Tsuji
  • ,
  • M Yamamoto
  • ,
  • S Iseki

68
5
開始ページ
1755
終了ページ
1763
記述言語
英語
掲載種別
DOI
10.1095/biolreprod.102.012344
出版者・発行元
SOC STUDY REPRODUCTION

Spermatogenic immunoglobulin superfamily (SgIGSF) is a mouse protein belonging to the immunoglobulin superfamily expressed in the spermatogenic cells of seminiferous tubules. We produced a specific polyclonal antibody against SgIGSF. Western blot analysis of the testes from postnatal developing mice using this antibody demonstrated multiple immunopositive bands of 80-130 kDa, which increased in number and size with the postnatal age. Enzymatic N-glycolysis caused reduction in the size of these bands to 70 kDa, indicating that SgIGSF is a glycoprotein and its glycosylation pattern and extent are developmentally regulated. Immunohistochemical analysis of the adult testis demonstrated that SgIGSF was present in the spermatogenic cells in the earlier steps of spermatogenesis and increased in amount from intermediate spermatogonia through zygotene spermatocytes but was diminished in the steps from early pachytene spermatocytes through round spermatids. After meiosis, SgIGSF reappeared in step 7 spermatids and was present in the elongating spermatids until spermiation. The immunoreactivity was localized primarily on the cell membrane. Consistent with the findings in adult testes, the analysis of the developing testes revealed that SgIGSF was expressed separately in the spermatogenic cells in earlier and later phases. Sertoli cells had no expression of SgIGSF, whereas both SgIGSF immunoprecipitated from the testis lysate and produced in COS-7 cells was shown to bind to the surface of Sertoli cells in primary culture. These results suggested that SgIGSF on the surface of spermatogenic cells binds to some membrane molecules on Sertoli cells in a heterophilic manner and thereby may play diverse roles in the spermatogenesis.

リンク情報
DOI
https://doi.org/10.1095/biolreprod.102.012344
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000182589700036&DestApp=WOS_CPL
ID情報
  • DOI : 10.1095/biolreprod.102.012344
  • ISSN : 0006-3363
  • Web of Science ID : WOS:000182589700036

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