MISC

2001年

Effects of mutation in hepatitis C virus nonstructural protein 5A on interferon resistance mediated by inhibition of PKR kinase activity in mammalian cells

MICROBIOLOGY AND IMMUNOLOGY
  • T Noguchi
  • ,
  • S Satoh
  • ,
  • T Noshi
  • ,
  • E Hatada
  • ,
  • R Fukuda
  • ,
  • A Kawai
  • ,
  • S Ikeda
  • ,
  • M Hijikata
  • ,
  • K Shimotohno

45
12
開始ページ
829
終了ページ
840
記述言語
英語
掲載種別
出版者・発行元
CENTER ACADEMIC PUBL JAPAN

The IFN-induced double-stranded RNA (dsRNA)-activated protein kinase PKR is one of the key molecules in the antiviral effects of IFN. To clarify the effects of hepatitis C virus nonstructural protein 5A (NS5A) on antiviral activity of IFN, in particular on PKR kinase activity, in mammalian cells, we established inducible NS5A-expressing cell lines derived from human osteosarcoma (Saos-2). The cells expressing NS5A derived from an IFN-resistant clone (NS5A-1b) that interacted with endogenous PYR in vitro, showed a suppressive effect on IFN function as determined by interference with vesicular stomatitis virus (VSV) infection, whereas NS5A (NS5A-2a) from an IFN-sensitive clone did not block the antiviral effect of IFN. A mutant with deletion of the IFN sensitivity determining region (ISDR) in NS5A-1b (NS5A-Delta ISDR) also interacted with PKR and suppressed its activity in vitro. However, neither NS5A-2a nor the C-terminal truncated mutant of NS5A-1b (NS5A-DeltaC) blocked PKR activity. These observations confirmed the previous report that the inhibitory effect of NS5A on IFN activity is mediated at least in part by the repression of PKR. In addition, we showed that IFN sensitivity was determined not only by the ISDR but that the involvement of the C-terminal region of NS5A-1b is important for the suppression of PKR activity.

Web of Science ® 被引用回数 : 44

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000172818600005&DestApp=WOS_CPL
ID情報
  • ISSN : 0385-5600
  • Web of Science ID : WOS:000172818600005

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