2014年12月
Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung
ONCOLOGY LETTERS
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 8
- 号
- 6
- 開始ページ
- 2561
- 終了ページ
- 2564
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3892/ol.2014.2572
- 出版者・発行元
- SPANDIDOS PUBL LTD
The insulin-like growth factor 2 gene (IGF2) is an imprinting gene, which mediates cell growth and apoptosis. The loss of imprinting (LOI) of IGF2 has been associated with the development of cancer. In the present study, loss LOI of IGF2 in lung cancer was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in combination with DNA sequencing of samples collected by laser capture microdissection. The status of each sample was assigned as imprinting when PCR-RFLP revealed only one band or sequence with a single peak; otherwise, the case was classified as LOI. LOI was identified in eight out of 13 adenocarcinoma cases (62%), but was not detected in any of the nine squamous cell carcinoma cases (0%). These results suggest that IGF2 LOI is involved in the molecular pathogenesis of lung adenocarcinoma, but not squamous cell carcinoma, and that LOI may be detected through increased IGF2 expression levels.
- リンク情報
- ID情報
-
- DOI : 10.3892/ol.2014.2572
- ISSN : 1792-1074
- eISSN : 1792-1082
- Web of Science ID : WOS:000345948600040