論文

査読有り 国際誌
2020年4月

A monoclonal antibody raised against a synthetic oxytocin peptide stains mouse hypothalamic neurones.

Journal of neuroendocrinology
  • Yuji Kogami
  • ,
  • Takahiro Tsuji
  • ,
  • Chiharu Tsuji
  • ,
  • Shigeru Yokoyama
  • ,
  • Kazumi Furuhara
  • ,
  • Olga Lopatina
  • ,
  • Anna Shabalova
  • ,
  • Alla B Salmina
  • ,
  • Yumi Watanabe
  • ,
  • Tsuyoshi Hattori
  • ,
  • Katsuhiko Nishimori
  • ,
  • Kota Kodama
  • ,
  • Haruhiro Higashida

32
4
開始ページ
e12815
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/jne.12815

A monoclonal antibody against oxytocin was generated in 7a5 hybridoma cells derived from myeloma cells and lymphocytes from the spleen of mice immunised with a synthetic oxytocin peptide. The 7a5 monoclonal antibody bound with oxytocin in enzyme-linked immunosorbent assays. 7a5 cell growth medium was diluted up to 5000-fold and used for immunohistochemistry. First, to test the specificity of the 7a5 antibody against oxytocin, we stained brain tissues of oxytocin knockout mice, comprising mice in which the first exon of the oxytocin-neurophysin gene is deleted. No 7a5 immunoreactivity was detected in the paraventricular nucleus (PVN) of the hypothalamus of oxytocin knockout mice; however, this area was strongly stained with the anti-vasopressin polyclonal antibody, HM07. Tissue preparations of the wild-type mouse PVN and supraoptic nucleus (SON) displayed 7a5 immunoreactivity that was indistinguishable from the staining produced with an anti-oxytocin polyclonal antibody, HM06. The immunoreactivity of HM06 in the PVN was similar to that of an anti-oxytocin monoclonal antibody, PS38. We then examined the cross-reactivity of 7a5 with arginine vasopressin. The majority of cell soma and processes stained by 7a5 were not co-stained with the vasopressin antibody in SON and PVN regions. Furthermore, the suprachiasmatic nucleus was stained by the vasopressin antibody but not by 7a5. These results demonstrate that 7a5 is a new anti-oxytocin monoclonal antibody recognising oxytocin and not vasopressin; therefore, 7a5 can be used to investigate the role of oxytocin in the brain.

リンク情報
DOI
https://doi.org/10.1111/jne.12815
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31770473
ID情報
  • DOI : 10.1111/jne.12815
  • ISSN : 0953-8194
  • PubMed ID : 31770473

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