MISC

2000年10月

Indole acetonitrile-sensitivity of transgenic tobacco containing Arabidopsis thaliana nitrilase genes

PLANT CELL REPORTS
  • M Dohmoto
  • ,
  • J Sano
  • ,
  • G Isaji
  • ,
  • K Yamaguchi

19
10
開始ページ
1027
終了ページ
1032
記述言語
英語
掲載種別
DOI
10.1007/s002990000218
出版者・発行元
SPRINGER-VERLAG

The Arabidopsis thaliana genome has four nitrilase (nitrile aminohydrolase, EC 3.5.5.1) genes (NIT1 to NIT4). These nitrilases catalyze hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). Growth of A. thaliana is inhibited by IAN probably due to hydrolysis of IAN to IAA, while the tobacco (Nicotiana tabacum) genome has only NIT4 homologs and is resistant-to IAN. In this study, we introduced A. thaliana NIT1 to NIT4 into tobacco. Introduction of NIT1, NIT2 or NIT3 into tobacco conferred growth inhibition by IAN. NIT2 transgenic plants were highly sensitive to IAN, and NIT1 and NIT3 transgenic plants were moderately sensitive. On the other hand, NIT4 transgenic plants were less sensitive to IAN, although some morphological changes in the roots were observed as the wild-type tobacco. These findings suggest that the ability of transgenic tobacco to convert IAN to IAA in vivo is markedly different among transgenes of NIT1 to NIT4.

リンク情報
DOI
https://doi.org/10.1007/s002990000218
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000090145900015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s002990000218
  • ISSN : 0721-7714
  • Web of Science ID : WOS:000090145900015

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