2006年11月
Liquid chromatography-mass spectrometric assay of androstenediol in prostatic tissue: Influence of androgen deprivation therapy on its level
STEROIDS
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- 巻
- 71
- 号
- 11-12
- 開始ページ
- 1007
- 終了ページ
- 1013
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.steroids.2006.08.003
- 出版者・発行元
- ELSEVIER SCIENCE INC
Androstenediol (Adiol, androst-5-ene-3 beta,17 beta-diol) is suspected of being an endogenous proliferation agent of prostate cancer (PCa) even after androgen deprivation therapy (ADT). A liquid chromatography-electron capture atmospheric pressure chemical ionization-mass spectrometric (LC-ECAPCI-MS) method for the determination of Adiol in prostatic tissue was developed and validated for evaluating the influence of ADT on the prostatic Adiol level. After derivatization of Adiol with 4-nitrobenzoyl chloride, the detection response of the derivative was increased 150 times more than that of intact Adiol. The LC-MS method was specific and reliable for the measurement of a trace amount of Adiol in 30 mg of tissue. The clinical study using the developed method showed that the prostatic Adiol level was not changed by ADT. That is, the prostatic Adiol levels of PCa patients with ADT (n = 12), benign prostate hypertrophy patients without ADT (n = 8) and bladder cancer patients (without prostatic disease) (n = 6) were 0.83 +/- 0.28, 0.62 +/- 0.31 and 0.71 +/- 0.28 ng g(-1) tissue, respectively, and there was no significant difference between these groups.
(C) 2006 Elsevier Inc. All rights reserved.
(C) 2006 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.steroids.2006.08.003
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018822929
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16978674
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242060200012&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.steroids.2006.08.003
- ISSN : 0039-128X
- CiNii Articles ID : 80018822929
- PubMed ID : 16978674
- Web of Science ID : WOS:000242060200012