Kuroda Akio

J-GLOBAL         Last updated: Sep 3, 2019 at 04:27
Kuroda Akio
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Hiroshima University
Research funding number

Awards & Honors

Oct 1995
"Human frontier science program, Long-term fellowship award."

Published Papers

Kuroda, Akio; Nishimura, Tomoki; Ishida, Takenori; Hirota, Ryuichi; Nomura, Kazutaka
BIOTECHNOLOGY AND BIOENGINEERING   99(2) 285-289   Feb 2008   [Refereed]
In the current studies, we found that the DksA protein from Escherichia coli binds strongly to chrysotile, which is the most commonly used form of asbestos. We developed a convenient colorimetric assay for chrysotile using a fusion of DksA and alk...
Ishida, Takenori; Alexandrov, Maxym; Nishimura, Tomoki; Minakawa, Kenji; Hirota, Ryuichi; Sekiguchi, Kiyoshi; Kohyama, Norihiko; Kuroda, Akio
ENVIRONMENTAL SCIENCE & TECHNOLOGY   44(2) 755-759   Jan 2010   [Refereed]
Fluorescence microscopy (FM) is one of the most important analytical tools in modern life sciences, sufficiently sensitive to allow observation of single molecules. Here we describe the first application of the FM technique for the detection of in...
The Si-tag for immobilizing proteins on a silica surface
Biotechnology and Bioengineering   96 1023-1029   Apr 2007   [Refereed]
The Si-tag for Immobilizing Proteins on a Silicon Device
Technical Proceedings of the 2007 Nanotechnology Conference and Trade Show   4 571-574   Mar 2007   [Refereed]
Kudo, Takashi; Kasama, Toshihiro; Ikeda, Takeshi; Hata, Yumehiro; Tokonami, Shiho; Yokoyama, Shin; Kikkawa, Takamaro; Sunami, Hideo; Ishikawa, Tomohiro; Suzuki, Masato et al.
JAPANESE JOURNAL OF APPLIED PHYSICS   48(6)    Jun 2009   [Refereed]
We fabricated a biosensor based on a silicon nanowire field-effect transistor (SiNW FET) with a Si(3)N(4) gate insulator for highly sensitive detection of target biomolecules. The fabricated SiNW FET acted as an ion-sensitive FET that could detect...
Ikeda, Takeshi; Ninomiya, Ken-ichi; Hirota, Ryuichi; Kuroda, Akio
PROTEIN EXPRESSION AND PURIFICATION   71(1) 91-95   May 2010   [Refereed]
We previously reported that a silica-binding protein, designated Si-tag, can be used as a fusion tag to immobilize functional proteins on silica surfaces. In this study, by taking advantage of the strong affinity of Si-tag for silica, we developed...
Kuroda, A; Nomura, K; Ohtomo, R; Kato, J; Ikeda, T; Takiguchi, N; Ohtake, H; Kornberg, A
SCIENCE   293(5530) 705-708   Jul 2001   [Refereed]
Inorganic polyphosphate (polyp), a polymer of hundreds of phosphate (P-i) residues, accumulates in Escherichia coli in response to stresses, including amino acid starvation. Here we show that the adenosine 5'-triphosphate-dependent protease Lon fo...
A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling
Biotechnol. Bioeng.   78 333-338   Apr 2001   [Refereed]
Motomura, Kei; Hirota, Ryuichi; Okada, Mai; Ikeda, Takeshi; Ishida, Takenori; Kuroda, Akio
APPLIED AND ENVIRONMENTAL MICROBIOLOGY   80(8) 2602-2608   Apr 2014   [Refereed]
Inorganic polyphosphate (polyP) is a linear polymer of tens to hundreds of phosphate (Pi) residues linked by high-energy" phosphoanhydride bonds as in ATP. PolyP kinases, responsible for the synthesis and utilization of polyP, are divided into two...
Use of an Escherichia coli recombinant producing thermostable polyphoaphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate
Applied and Environmental Microbiology   73(17) 5676-5678   Sep 2007   [Refereed]
ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell
Biosci. Biotech. Biochem.   68 1216-1220   Apr 2004   [Refereed]
Increase in bioluminescence intensity of firefly luciferase using genetic modification
Analytical Biochemistry   366 131-136   Jul 2007   [Refereed]
Noda, Kenichi; Goto, Hitoshi; Murakami, Yuji; Ahmed, Abo Bakr F.; Kuroda, Akio
ANALYTICAL BIOCHEMISTRY   397(2) 152-155   Feb 2010   [Refereed]
The Limulus reaction is an application of the defense mechanism of horseshoe crab for endotoxin detection. Endotoxin is a component of the cell wall in the outer membrane of gram-negative bacteria, and causes fever or shock when it enters the huma...
Hirota, Ryuichi; Yamane, Sho-taro; Fujibuchi, Tatsuya; Motomura, Kei; Ishida, Takenori; Ikeda, Takeshi; Kuroda, Akio
JOURNAL OF BIOSCIENCE AND BIOENGINEERING   113(4) 445-450   Apr 2012   [Refereed]
Phosphite dehydrogenase (PtxD), which catalyzes the nearly irreversible oxidation of phosphite to phosphate with the concomitant reduction of NAD(+) to NADH, has great potential for NADH regeneration in industrial biocatalysts. Here, we isolated a...
Ikeda, Takeshi; Hata, Yumehiro; Ninomiya, Ken-ichi; Ikura, Yoshiaki; Takeguchi, Keigo; Aoyagi, Satoka; Hirota, Ryuichi; Kuroda, Akio
ANALYTICAL BIOCHEMISTRY   385(1) 132-137   Feb 2009   [Refereed]
We previously reported a silica-binding protein, designated Si-tag, which can be used to immobilize proteins on silica surfaces. Here, we constructed a fusion protein of Si-tag and immunoglobulin-binding staphylococcal protein A for oriented immob...
Ishida, Takenori; Ikeda, Tsukasa; Takiguchi, Noboru; Kuroda, Akio; Ohtake, Hisao; Kato, Junichi
APPLIED AND ENVIRONMENTAL MICROBIOLOGY   73(10) 3183-3188   May 2007   [Refereed]
N-Octanoyl cyclopentylamide (C8-CPA) was found to moderately inhibit quorum sensing in Pseudomonas aeruginosa PAO1. To obtain more powerful inhibitors, a series of structural analogs of C8-CPA were synthesized and examined for their ability to inh...
Satoh, Tetsuya; Tsuruta, Kosuke; Shinoda, Yasuharu; Hirota, Ryuichi; Noda, Kenichi; Kuroda, Akio; Murakami, Yuji
ANALYTICAL BIOCHEMISTRY   395(2) 161-165   Dec 2009   [Refereed]
We constructed a novel ATP amplification reactor using a reciprocating-flow system to increase the number of ATP amplification cycles without an increase in backpressure. We previously reported a continuous-flow ATIP amplification system that effe...
Satoh, Tetsuya; Shinoda, Yasuharu; Tokonami, Shiho; Hirota, Ryuichi; Noda, Kenichi; Kuroda, Akio; Murakami, Yuji
SENSORS AND ACTUATORS B-CHEMICAL   142(1) 118-122   Oct 2009   [Refereed]
In this study, we constructed a novel microfluidic device for continuous-flow ATP amplification, using the SU-8:PDMS method. Sepharose beads immobilized with adenylate kinase and pyruvate kinase was packed into a microfluidic chamber to form lamin...
Fukuyama, Masataka; Yamatogi, Shinya; Ding, Hao; Nishida, Mizue; Kawamoto, Chika; Amemiya, Yoshiteru; Ikeda, Takeshi; Noda, Tomohide; Kawamoto, Seiji; Ono, Kazuhisa et al.
JAPANESE JOURNAL OF APPLIED PHYSICS   49(4)    2010   [Refereed]
Si ring optical resonators have been fabricated by the Si LSI technology using electron beam lithography and reactive ion etching. The resonator is applied for sensing receptor-analyte reaction using silicon-binding protein, Si-tag, which enables ...
Kashihara, Hitoshi; Kang, Bong Mun; Omasa, Takeshi; Honda, Kohsuke; Sameshima, Yuka; Kuroda, Akio; Ohtake, Hisao
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74(4) 865-868   Apr 2010   [Refereed]
Heat treatment is a simple technique for releasing polyphosphate (polyP) from microbial cells. We investigated the ultrastructural alterations of polyP granules (PPGs) in response to heat treatment at 70 degrees C using a polyP-accumulating phoU m...

Books etc

Molecular genetics of bacterial polyphosphate accumulation to better understand the mechanism underlying biological phosphorus removal.
Applications of polyphosphate and other biopolmers in wastewater treatment.
New current of biotechnology
Feb 1999   ISBN:4254171064
Chemotactic signal transduction network in Pseudomonas aeruginosa
Molecular Biology of Pseudomonads. American Society for Microbiology= Washington USA   1996   
Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater
Inorganic polyphosphates   1999