論文

査読有り
2018年6月1日

Acceleration of nucleation of prion protein during continuous ultrasonication

Journal of Biochemistry
  • Kei-Ichi Yamaguchi
  • ,
  • Ryo P Honda
  • ,
  • Abdelazim Elsayed Elhelaly
  • ,
  • Kazuo Kuwata

163
6
開始ページ
503
終了ページ
513
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jb/mvy015
出版者・発行元
Oxford University Press

Although pulsatile irradiation of ultrasonication is frequently used for generating amyloid fibrils in vitro, the potential for inducing amyloid fibrillation of proteins during continuous ultrasonication is unknown. In this study, we implemented a continuous irradiation system and measured far-ultraviolet circular dichroism in a real-timemanner.During the continuous ultrasonication, the conformation of full-length mouse prion protein (mPrP) was rapidly altered without a lag time and electron microscopy revealed that distorted fibrils, β-oligomers and amorphous aggregates were formed at pH 2.2, 4.0 and 9.1, respectively. Similarly, hen egg white lysozyme formed distorted fibrils and small and large amorphous aggregates atpH2.2 and 7.1 and 11.9, respectively, without a lag time. The concentration dependencies of the initial rates were different between the two systems. The aggregate formation of mPrPfollowed a first-order reaction, whereas that of lysozyme followed the zeroth-order reaction. Importantly, the reactions were immediately stopped by switching off ultrasonication, and restarted instantaneously when ultrasonication was restarted. Thus, the continuous ultrasonication significantly accelerates the nucleations of mPrP and lysozyme aggregates by the interaction between monomer and cavitation bubble. These cavitation bubbles may act as catalysts that decrease the activation free energy for nucleation, which is low in mPrP and high in lysozyme.

リンク情報
DOI
https://doi.org/10.1093/jb/mvy015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29409004
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000434087500008&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048698329&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85048698329&origin=inward
ID情報
  • DOI : 10.1093/jb/mvy015
  • ISSN : 1756-2651
  • ISSN : 0021-924X
  • eISSN : 1756-2651
  • PubMed ID : 29409004
  • SCOPUS ID : 85048698329
  • Web of Science ID : WOS:000434087500008

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