J-GLOBAL         Last updated: Jul 2, 2019 at 02:42
Gifu University
Graduate School of Medicine
(BLANK)(Gifu University)


Antiaging medicine/Cell death and survival/Neuronal cell differentiation/Cancer cell apoptosis/Alcohol metabolism

Research Areas


Academic & Professional Experience

Gifu University School of Medicine, Medical Course

Published Papers

Tawada Masahiro, Hayashi Shin-ichiro, Osada Shinji, Nakashima Shigeru, Yoshida Kazuhiro
Journal of gastroenterology   47(9) 1057-1060   Sep 2012   [Refereed]
Lymphatic metastasis is a critical determinant of prognosis in human gastrointestinal cancers. Studies suggest that lymphatic metastasis has been linked to lymphangiogenesis, the growth of lymphatic vessels, while the mechanisms of tumor lymphangi...
Kozawa Sachi, Honda Ayako, Kajiwara Naomi, Takemoto Yasuhiko, Nagase Tomoko, Nikami Hideki, Okano Yukio, Nakashima Shigeru, Shimozawa Nobuyuki
Molecular medicine reports   4(6) 1157-1162   [Refereed]
Peroxisomes catalyze a range of essential metabolic functions, mainly related to lipid metabolism. However, their roles in obesity have yet to be clarified. The aim of this study was to investigate the correlation between obesity and peroxisomal l...
Ikegame Yuka, Yamashita Kentaro, Hayashi Shin-Ichiro, Mizuno Hiroshi, Tawada Masahiro, You Fukka, YamadaKiyofumi, Tanaka Yoshitaka, Egashira Yusuke, Nakashima Shigeru, Yoshimura Shin-Ichi, Iwama Toru
Cytotherapy   13(6) 675-685   Jul 2011   [Refereed]
Transplantation of mesenchymal stromal cells (MSC) derived from bone marrow (BM) or adipose tissue is expected to become a cell therapy for stroke. The present study compared the therapeutic potential of adipose-derived stem cells (ASC) with that ...
Ikegame Yuka, Yamashita Kentaro, Hayashi Shin-ichiro, Yoshimura Shin-ichi, Nakashima Shigeru, Iwama Toru
Hypertension research : official journal of the Japanese Society of Hypertension   33(7) 703-707   Jul 2010   [Refereed]
Release of neutrophil elastase is one of the harmful inflammatory reactions in acute cerebral ischemia. Therefore, inhibition of elastase released from neutrophils could be a useful strategy for the treatment of acute stroke. To evaluate this hypo...
Hayashi Shin-ichiro, Sato Naoyuki, Yamamoto Akitsugu, Ikegame Yuka, Nakashima Shigeru, Ogihara Toshio, Morishita Ryuichi
Arteriosclerosis, thrombosis, and vascular biology   29(11) 1909-1915   Nov 2009   [Refereed]
Although the majority of cases of Alzheimer disease (AD) are known to be attributable to the sporadic (nongenetic) form of the disease, the mechanism underlying its cause and progression still remains unclear.We found that vascular beta-amyloid (A...


Motoshi Sawada, Shigeru Nakashima, Tohru Kiyono, Jun Yamada, Shigeru Hara, Masanori Nakagawa, Jun Shinoda, Noboru Sakai
Experimental Cell Research   273 157-168
During apoptosis of human glioma cells induced by anti-Fas antibody, ceramide formation with activation of acid, but not neutral sphingomyelinase (SMase), was observed. A potent inhibitor of acid SMase, SR33557, effectively inhibited ceramide form...
H. Yamakawa, Y. Ito, T. Naganawa, Y. Banno, S. Nakashima, S. I. Yoshimura, M. Sawada, Y. Nishimura, Y. Nozawa, N. Sakai
Neurological Research   22 556-564   Jan 2000
The treatment of PC12 cells with H2O2 (100-500 μM) resulted in typical apoptotic changes including fragmentation and condensation of nuclei, and DNA fragmentation observed as DNA ladder. H2O2-induced apoptosis was associated with activation of cas...
Activation of caspase-9 and-3 during H2O2-induced apoptosis of PC12 cells independnent of ceramide formation.
Neurol Res   22 556-564   2000
Fukka You, Kenta Aoki, Yatsuji Ito, Shigeru Nakashima
Molecular Medicine Reports   2 609-613
5-Fluorouracil (5-FU) is a widely used chemotherapeutic agent that inhibits the growth and initiates the apoptosis of epithelial tumors, including squamous cell carcinoma of the head and neck region. However, resistance to this drug is often obser...

Books etc

Cellular signal transduction ; phospholipase C.
Biosignal Transduction   1991   
Role and significance of membrane phospholipid metabolism.
Current biomembrane system   1991   
Role of signal-transducing phospholipase A2, C and D in platelet activation.
Advances in Molecular and Cell Biology Series "The Platelet" JAI Press Inc.   1996   
Platelet phospholipase C, D.
Handbook of Experimental Pharmacology "Platelets and Their Factors" Springer-Verlag   1996   
Bradykinin B2 receptors and signal transduction analyzed in NG108-15 neuroblastoma x glioma hybrid cells, B2 receptor-transformed CHO cells and rastransformed NIH3T3 fibroblasts.
Progress in Brain Reserch Elsevier Science Publisher B. V.   1996   


Molecular mechanism of ceramide-mediafed apoptosis
Molecular mechanism of neuronal cell death
Apoptosis by natural telpenoids

Research Grants & Projects

Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 1998 - 1999    Investigator(s): Shigeru NAKASHIMA
Phospholipase D (PLD) hydrolyzes phospholipids, especially phosphatidylcholine (PC) to phosphatidic acid (PA) and choline. PA acts as a second messenger and can be converted to other messenger molecules, such as lysophosphatidic acid (LPA) and 1,2...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 1996 - 1997    Investigator(s): Shigeru NAKASHIMA
We have found the alternatively spliced isoform of human phospholipase D1 (hPLD1). Moreover, the entire coding regions of rat PLD1 and PLD2 were isolated and sequenced. A shorter alternatively spliced form of rPLD1 (rPLD1b) was also identified. Ch...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(一般研究(C))
Project Year: 1994 - 1995    Investigator(s): Shigeru NAKASHIMA
The combination of beta-glycerophosphate and ascorbic acid increased alkaline phosphate (ALP) activity, a maraker for osteoblastic differentiation, in clonal osteoblast like cell line MC3T3-E1. The increase was detected as 24 hours after the addit...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2000 - 2001    Investigator(s): Shigeru NAKASHIMA
The gene encoding phospholipase D (PLD) of Caenorhabditis elegans (pld-1) was isolated. The isolated cDNA encodes a 1,427 amino acid protein which contains four conserved regions defined by the primary structures of bacterial, plant, yeast and mam...
Molecular mechanism of signal transducing phospholipose activation