2001年3月
Glucocorticoid-induced insulin resistance associates with activation of protein kinase C isoforms
CELLULAR SIGNALLING
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- 巻
- 13
- 号
- 3
- 開始ページ
- 169
- 終了ページ
- 175
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0898-6568(01)00143-7
- 出版者・発行元
- ELSEVIER SCIENCE INC
We studied glucocorticoid-induced insulin resistance and possible role of protein kinase C (PKC). Pretreatment with dexamethasone, prednisolone and corticosterone for 60 min decreased insulin-induced [H-3] 2-deoxyglucose (DOG) uptake in isolated rat adipocytes. Preincubation with Go6976, LY379196 or myristoylated PKC pseudosubstrate. conventional PKC inhibitor, but not cycloheximide or RU38486, recovered dexamethasone-induced insulin resistance. Dexamethasone activated immunoprecipitates with anti-PKC alpha, beta, and zeta antibodies. PKC zeta activity in adipocytes increased to 163%. and 264% from basal level (100%) with dexamethasone and insulin treatment, respectively. Dexamethasone provoked redistribution of both PKC beta and zeta from the cytosol to the membrane. These results indicate that dexamethasone activates both conventional and atypical PKC. However, conventional PKC is more important in glucocorticoid-induced insulin resistance. (C) 2001 Elsevier Science Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/S0898-6568(01)00143-7
- CiNii Articles
- http://ci.nii.ac.jp/naid/80012366671
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11282455
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000167933500003&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/S0898-6568(01)00143-7
- ISSN : 0898-6568
- CiNii Articles ID : 80012366671
- PubMed ID : 11282455
- Web of Science ID : WOS:000167933500003