SUGIYAMA Makoto

J-GLOBAL         Last updated: Jul 2, 2019 at 02:42
 
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Name
SUGIYAMA Makoto
Affiliation
Gifu University
Section
Faculty of Applied Biological Sciences
Degree
Ph.D. in Agriculture(The University of Tokyo), (BLANK)

Profile

Research on control of viral zoonotic diseases/To control of viral zoonotic diseases caused by rotavirus, rabies virus and other viruses, I have performed the research on pathogenisity and epidemiology of these viruses and development of diagnosis and treatment for these diseases.

Research Areas

 
 

Academic & Professional Experience

 
1987
 - 
1995
Research Associate, Faculty of Agriculture, Gifu University
 
1995
 - 
2004
Associate Professor, Faculty of Agriculture, Gifu University
 
2004
 - 
2007
Professor, United Graduate School of Veterinary Sciences, Gifu University
 
2007
   
 
- Professor, Faculty of Applied Biological Sciences, Gifu University
 

Education

 
 
 - 
1981
Faculty of Agriculture, Gifu University
 
 
 - 
1983
Graduate School of Agricultural Science, Graduate School, Division of Agriculture, Gifu University
 

Published Papers

 
Anindita, P. D, Sasaki, M., Okada, K., Ito, N., Sugiyama, M., Saito-Tarashima, N., Minakawa, N., Shuto, S., Otsuguro, S., Ichikawa, S., Matsuda, A., Maenaka, K., Orba, Y., Sawa, H.
Antiviral Res.   S0166-3542(17)30854-9.    Mar 2018   [Refereed]
Generation of recombinant rotaviruses expressing fluorescent proteins using an optimized reverse genetics system
Satoshi Komoto, Saori Fukuda, Tomihiko Ide, Naoto Ito, Makoto Sugiyama, Tetsushi Yoshikawa, Takayuki Murata, and Koki Taniguchi
J. Vitol.      2018   [Refereed]
Heparan sulfate proteoglycans serve as an attachment factor for rabies virus entry and infection.
Sasaki, M., Anindita, P. D., Ito, N., Sugiyama, S., Carr, M., Fukuhara, H., Ose, T., Maenaka, K., Takada, A., Hall, W. W., Orba, Y., and Sawa, H.
J. Infect. Dis.      2018   [Refereed]
Yamaoka S., Okada K., Ito N., Okadera K., Mitake H., Nakagawa K. and Sugiyama M.
J. Vet. Med. Sci.   79(8) 1394-1397   2017   [Refereed]
Molecular function analysis of rabies virus RNA polymerase L protein by using an L gene-deficient virus.
Nakagawa K., Kobayashi Y., Ito N., Suzuki Y., Okada K., Makino M., Goto H., Takahashi T. and Sugiyama M.
J. Virol.   91(20) e00826-17   2017   [Refereed]
Nakagawa K., Nakagawa K., Mitake H., Okada K., Yamaoka S., Takashima Y., Masatani T., Okadera K., Ito N. and Sugiyama M.
Vaccine   35 5622-5628   2017   [Refereed]
Okada K., Ito N., Yamaoka, S., Masatani, T., Ebihara, H., Goto, H., Nakagawa, K., Mitake, H., Okadera, K. and Sugiyama, M.
J. Virol.   90(18) 8226-8237   2016   [Refereed]
Ogino, M., Ito, N., Sugiyama, M. and Ogino, T.
Viruses   8(5) 144   2016   [Refereed]
Mitake, H., Fujii, Y., Nagai, M., Ito, N., Okadera, K., Okada, K., Nakagawa, K., Kishimoto, M., Mizutani, T. Okazaki, K., Sakoda, Y., Takada, A. and Sugiyama, M.
J. Gen. Virol.   97(8) 1818-1822   2016   [Refereed]
Okadera, K., Abe, M., Ito, N., Mitake, H., Okada, K., Nakagawa, K., Une, Y., Tsunemitsu, H. and Sugiyama, M.
J. Gen. Virol.   97(5) 1158-1167   2016   [Refereed]
Anindita, P. D., Sasaki, M., Nobori, H., Sato, A., Carr, M., Ito, N., Sugiyama, M., Orba, Y., Sawa, H.
Virus Res.   215 121-128   2016   [Refereed]
Genome Sequences of Rotavirus A Strains Ty-1 and Ty-3, Isolated from Turkeys in Ireland in 1979.
Fujii, Y., Mitake, H., Yamada, D., Nagai, M., Okadera, K., Ito, N., Okada, K., Nakagawa, K., Mizutani, T. and Sugiyama, M.
Genome Announc.   4(1) e01565-15   2016   [Refereed]
Masatani, T., Ozawa, M., Yamada, K., Ito, N., Horie, M., Matuu, A., Okuya, K., Tsukiyama-Kohara, K., Sugiyama, M., Nishizono, A.
J. Gen. Virol.   97(2) 316-326   2016   [Refereed]
Hiromichi Mitake, Naoto Ito, Naoto Ito, Kota Okadera, Kazuma Okada, Kento Nakagawa, Tomomi Tanaka, Kiyohito Katsuragi, Kasumi Kasahara, Toshihide Nihongi, Shoji Sakurai, Hiroshi Tsunemitsu, Hiroshi Tsunemitsu, Makoto Sugiyama, Makoto Sugiyama
Journal of General Virology   96(9) 2708-2713   2015
© 2015 The Authors. Previous studies revealed that rotavirus A (RVA) is present in not only the small intestine but also various organs. It was reported that RVA persisted in mesenteric lymph nodes (MLNs) in experimental models. However, there hav...
Hiromichi Mitake, Naoto Ito, Naoto Ito, Kota Okadera, Kazuma Okada, Kento Nakagawa, Tomomi Tanaka, Kiyohito Katsuragi, Kasumi Kasahara, Toshihide Nihongi, Hiroshi Tsunemitsu, Hiroshi Tsunemitsu, Makoto Sugiyama, Makoto Sugiyama
Journal of Veterinary Medical Science   77(2) 221-224   2015
©2015 The Japanese Society of Veterinary Science A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011–2012 were examined by RT-seminested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLA...
Linda Wiltzer, Linda Wiltzer, Kazuma Okada, Satoko Yamaoka, Florence Larrous, Florence Larrous, Henna Veera Kuusisto, Makoto Sugiyama, Danielle Blondel, Hervé Bourhy, David Andrew Jans, Naoto Ito, Gregory William Moseley, Gregory William Moseley
Journal of Infectious Diseases   209(11) 1744-1753   2014
Background. Rabies virus (RABV) causes rabies disease resulting in >55 000 human deaths/year. The multifunctional RABV P-protein has essential roles in genome replication, and forms interactions with cellular STAT proteins that are thought to unde...
A single immunization with recombinant rabies virus (ERAG3G) confers complete protection against rabies in mice.
Yang, D. K., Nakagawa, K., Ito, N., Kim, H. H., Hyun, B. H., Nah, J. J., Sugiyama, M. and Song, J. Y.
Clin. Exp. Vaccine Res.   3 176-184   2014   [Refereed]
Satoko Yamaoka, Naoto Ito, Naoto Ito, Seii Ohka, Seii Ohka, Shohei Kaneda, Shohei Kaneda, Hiroko Nakamura, Hiroko Nakamura, Takahiro Agari, Tatsunori Masatani, Tatsunori Masatani, Keisuke Nakagawa, Kazuma Okada, Kota Okadera, Hiromichi Mitake, Teruo Fujii, Teruo Fujii, Makoto Sugiyama, Makoto Sugiyama
Journal of Virology   87(22) 12327-12338   Jan 2013
Rabies virus (RABV), which is transmitted via a bite wound caused by a rabid animal, infects peripheral nerves and then spreads to the central nervous system (CNS) before causing severe neurological symptoms and death in the infected individual. D...
Kota Okadera, Masako Abe, Naoto Ito, Naoto Ito, Shigeki Morikawa, Ari Yamasaki, Tatsunori Masatani, Keisuke Nakagawa, Satoko Yamaoka, Makoto Sugiyama, Makoto Sugiyama
Infection, Genetics and Evolution   20 54-60   Jan 2013
Group A rotaviruses (RVAs) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. RVAs have also been detected in several wild and zoo animals, indicating wide susceptibility of wild animals. However, the role of w...
Tatsunori Masatani, Tatsunori Masatani, Naoto Ito, Naoto Ito, Yuki Ito, Keisuke Nakagawa, Masako Abe, Satoko Yamaoka, Kota Okadera, Makoto Sugiyama, Makoto Sugiyama
Microbiology and Immunology   57(7) 511-517   2013
By using a cultured neuroblastoma cell line, the present authors recently showed that the N protein of virulent rabies virus fixed strain Nishigahara (Ni), but not that of the attenuated derivative Ni-CE, mediates evasion of induction of type I in...
Masaharu Fukuda, Masaharu Fukuda, Masaharu Fukuda, Kazufumi Kuga, Kazufumi Kuga, Ayako Miyazaki, Tohru Suzuki, Keito Tasei, Tsunehiko Aita, Masaji Mase, Masaji Mase, Makoto Sugiyama, Hiroshi Tsunemitsu, Hiroshi Tsunemitsu
Archives of Virology   157(6) 1063-1069   2013
A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR...
Keisuke Nakagawa, Naoto Ito, Naoto Ito, Tatsunori Masatani, Masako Abe, Satoko Yamaoka, Yuki Ito, Kota Okadera, Makoto Sugiyama, Makoto Sugiyama
Vaccine   30(24) 3610-3617   2012
An amino acid substitution at position 333 in rabies virus G protein is known to determine the pathogenicity: strains with Arg or Lys at that position kill adult mice after intracerebral inoculation, whereas strains with other amino acids cause no...
Masako Abe, Naoto Ito, Naoto Ito, Tatsunori Masatani, Keisuke Nakagawa, Satoko Yamaoka, Yoshihiro Kanamaru, Hiroto Suzuki, Ken Ichi Shibano, Yasuhiro Arashi, Yasuhiro Arashi, Makoto Sugiyama, Makoto Sugiyama
Journal of General Virology   92(4) 952-960   Apr 2011
We have reported a novel bovine rotavirus, the AzuK-1 (G21P[29]) strain, isolated from an asymptomatic calf. We isolated another bovine rotavirus, the Dai-10 strain, bearing new G24P[33] genotypes, assigned by the Rotavirus Classification Working ...
Tatsunori Masatani, Naoto Ito, Naoto Ito, Kenta Shimizu, Kenta Shimizu, Yuki Ito, Keisuke Nakagawa, Masako Abe, Satoko Yamaoka, Makoto Sugiyama, Makoto Sugiyama
Virus Research   155(1) 168-174   Jan 2011
We previously reported that nucleoprotein (N) is related to the different pathogenicities of the virulent rabies virus strain Nishigahara (Ni) and avirulent strain Ni-CE and also that Ni N, but not Ni-CE N, functions to evade retinoic acid-inducib...
Kentaro Yoshii, Keita Mottate, Yuki Omori-Urabe, Yumiko Chiba, Takahiro Seto, Takahiro Sanada, Junko Maeda, Mayumi Obara, Shuji Ando, Shuji Ando, Naoto Ito, Makoto Sugiyama, Hiroshi Sato, Hiroshi Fukushima, Hiroaki Kariwa, Ikuo Takashima
Journal of Veterinary Medical Science   73(3) 409-412   2011
Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. Rodent species that are potential hosts for TBEV are widely distributed in various regions in Japan. In this study, we carried out large-scale epizooti...
Naoto Ito, Naoto Ito, Tetsuo Mita, Kenta Shimizu, Yuki Ito, Tatsunori Masatani, Keisuke Nakagawa, Satoko Yamaoka, Masako Abe, Kota Okadera, Nobuyuki Minamoto, Nobuyuki Minamoto, Makoto Sugiyama, Makoto Sugiyama
Journal of Veterinary Medical Science   73(10) 1363-1366   2011
We previously reported that rabies virus strain CE(NiM), but not the parental Ni-CE strain, killed mice after intracerebral inoculation. CE(NiM) and Ni-CE are genetically identical except for two amino acids at positions 29 and 95 in the M protein...
Masako Abe, Naoto Ito, Kouji Sakai, Yoshihiro Kaku, Mami Oba, Miho Nishimura, Ichiro Kurane, Masayuki Saijo, Shigeru Morikawa, Makoto Sugiyama, Tetsuya Mizutani
Virus Genes   43(2) 243-248   2011
A novel sapelovirus-like virus was isolated from a wild boar (Sus scrofa). In this study, partial viral genomic nucleotide sequences were determined using the rapid determination system of viral nucleic acid sequences (RDV) ver. 3.1, which we rece...
Naoto Ito, Naoto Ito, Gregory W. Moseley, Danielle Blondel, Kenta Shimizu, Caitlin L. Rowe, Yuki Ito, Tatsunori Masatani, Keisuke Nakagawa, David A. Jans, Makoto Sugiyama, Makoto Sugiyama
Journal of Virology   84(13) 6699-6710   Jul 2010
The fixed rabies virus (RV) strain Nishigahara kills adult mice after intracerebral inoculation, whereas the chicken embryo fibroblast cell-adapted strain Ni-CE causes nonlethal infection in adult mice. We previously reported that the chimeric CE(...
Tatsunori Masatani, Naoto Ito, Naoto Ito, Kenta Shimizu, Yuki Ito, Keisuke Nakagawa, Yoshiharu Sawaki, Hiroyuki Koyama, Makoto Sugiyama, Makoto Sugiyama
Journal of Virology   84(8) 4002-4012   Apr 2010
The rabies virus Ni-CE strain causes nonlethal infection in adult mice after intracerebral inoculation, whereas the parental Nishigahara (Ni) strain kills mice. We previously reported that the chimeric CE(NiN) strain with the N gene from the Ni st...
Masako Abe, Ari Yamasaki, Naoto Ito, Naoto Ito, Toshio Mizoguchi, Makoto Asano, Tsukasa Okano, Makoto Sugiyama, Makoto Sugiyama
Veterinary Microbiology   146(3-4) 253-259   2010
Group A rotaviruses infect and cause diarrhea in humans and a wide range of mammals. Previous studies have suggested that some strains can cross the species barrier to infect humans (Martella et al., 2010). However, there are few reports on infect...
Yuki Ito, Naoto Ito, Naoto Ito, Shouichiro Saito, Shouichiro Saito, Tatsunori Masatani, Keisuke Nakagawa, Yasuro Atoji, Yasuro Atoji, Makoto Sugiyama, Makoto Sugiyama
Microbiology and Immunology   54(2) 89-97   2010
Rabies virus Nishigahara strain kills adult mice after intracerebral inoculation, hereas the derivative RC-HL strain does not. It has previously been reported by us that the R(G 242/255/268) strain, in hich amino acids at positions 242, 255 and 26...
Kazufumi Kuga, Kazufumi Kuga, Ayako Miyazaki, Takako Suzuki, Michihiro Takagi, Nachiko Hattori, Ken Katsuda, Masaji Mase, Masaji Mase, Makoto Sugiyama, Hiroshi Tsunemitsu, Hiroshi Tsunemitsu
Archives of Virology   154(11) 1785-1795   Oct 2009
We determined the nucleotide sequences of the outer capsid glycoprotein (VP7) genes of 38 porcine group B rotaviruses (GBRs) from feces of pigs at 27 farms in Japan between 2000 and 2007. Substantial diversity among porcine GBR VP7 genes was obser...
Masako Abe, Naoto Ito, Naoto Ito, Shigeki Morikawa, Masaki Takasu, Tetsuma Murase, Takanori Kawashima, Yoshihiro Kawai, Junko Kohara, Makoto Sugiyama, Makoto Sugiyama
Virus Research   144(1-2) 250-257   2009
A total of 171 fecal specimens collected from healthy calves on a beef farm in Gifu Prefecture, Japan in 2006-2007 were examined for group A rotaviruses by RT-semi-nested PCR targeting the coding region for VP8*. Nine specimens were positive for r...
S. Nakamura, T. Okano, H. Shibata, M. Saito, T. Komatsu, M. Asano, M. Sugiyama, T. Tsubota, M. Suzuki
Canadian Journal of Zoology   86(9) 1042-1049   Sep 2008
As a first step to study the relationship between fat accumulation and reproductive success in Japanese black bears (Ursus thibetanus japonicus Schlegel, 1857) with the focus on leptin, we determined leptin cDNA sequences in the bears. Next, we st...
Heru Susetya, Makoto Sugiyama, Makoto Sugiyama, Akio Inagaki, Naoto Ito, Naoto Ito, Gatot Mudiarto, Nobuyuki Minamoto, Nobuyuki Minamoto
Virus Research   135(1) 144-149   Jul 2008
In order to clarify the genetic relationships and dynamics of rabies viruses that are epidemic in Indonesia, we determined and analyzed 1307 nucleotides of nucleoprotein genes of 34 rabies field isolates collected from Sumatra, Java, Kalimantan, S...
Nakamura, S. Okano, T., Yoshida, Y., Matsumoto, A., Murase, Y., Kato, H., Komatsu, T., Asano, M., Suzuki, M., Sugiyama, M. & Tusbota, T.
Jpn. J. Zoo, Woldl. Med.   13(1) 15-20   2008
Tetsuo Mita, Kenta Shimizu, Naoto Ito, Naoto Ito, Kentaro Yamada, Yuki Ito, Makoto Sugiyama, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Virus Research   137(1) 33-39   2008
The molecular mechanism involved in cytopathogenicity of rabies virus has not been fully elucidated yet. A fixed rabies virus Nishigahara strain does not induce clear cytopathic effect (CPE) in mouse neuroblastoma (NA) cells, whereas Ni-CE strain,...
Kenta Shimizu, Naoto Ito, Naoto Ito, Tetsuo Mita, Kentaro Yamada, Junji Hosokawa-Muto, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Virus Research   123(2) 154-160   2007
Rabies virus Ni-CE strain causes nonlethal infection in adult mice after intracerebral inoculation, whereas the parental Nishigahara strain kills mice. In this study, to identify viral gene(s) related to the difference in pathogenicity between Ni-...
Mohamed A. Borghan, Yoshio Mori, Abu Baker El-Mahmoudy, Naoto Ito, Makoto Sugiyama, Tadashi Takewaki, Nobuyuki Minamoto
Journal of General Virology   88(7) 2064-2072   2007
Rotavirus non-structural protein (NSP) 4 can induce aqueous secretion in the gastrointestinal tract of neonatal mice through activation of an age- and Ca2+-dependent plasma membrane anion permeability. Accumulating evidence suggests that nitric ox...
Kenta Shimizu, Naoto Ito, Naoto Ito, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   50(12) 975-978   2006
The growth of a virulent strain of fixed rabies virus, Nishigahara, in mouse neuroblastoma NA cells treated with type I interferon (IFN) was compared with that of a derivative avirulent strain, Ni-CE. Nishigahara strain was slightly sensitive to I...
Mutsuyo Takayama-Ito, Mutsuyo Takayama-Ito, Naoto Ito, Naoto Ito, Kentaro Yamada, Makoto Sugiyama, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Virus Research   115(2) 169-175   2006
We have reported that the region between amino acids 164 and 303 in the glycoprotein of rabies Nishigahara strain is important for lethality in adult mice. The region contains nine amino acid substitutions between the virulent Nishigahara and the ...
Kentaro Yamada, Naoto Ito, Naoto Ito, Mutsuyo Takayama-Ito, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   50(1) 25-32   2006
Rabies virus Nishigahara strain causes lethal infection in adult mice after intracerebral inoculation. On the other hand, the RC-HL strain, derived from the Nishigahara strain, does not cause lethal infection in adult mice. We previously demonstra...
Junji Hosokawa-Muto, Naoto Ito, Naoto Ito, Kentaro Yamada, Kenta Shimizu, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   50(3) 187-196   2006
A recombinant rabies virus carrying double glycoprotein (G) genes, R(NPMGGL) strain, was generated by a reverse genetics system utilizing cloned cDNA of the RC-HL strain, and the biological properties of the virus were compared to those of the rec...
Naoto Ito, Makoto Sugiyama, Kentaro Yamada, Kenta Shimizu, Mutsuyo Takayama-Ito, Junji Hosokawa, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   49 (11), 971-979 971-979   2005
Matrix (M) protein of rabies virus is known to play an important role in assembly and budding of the progeny virus. We generated an M gene-deficient rabies virus, RC-HLΔM, using a reverse genetics system of rabies virus RC-HL strain to develop a n...
Mutsuyo Takayama-Ito, Naoto Ito, Naoto Ito, Kentaro Yamada, Nobuyuki Minamoto, Nobuyuki Minamoto, Makoto Sugiyama, Makoto Sugiyama
Journal of NeuroVirology   10(2) 131-135   2004
The authors have previously reported that the glycoprotein of the pathogenic Nishigahara strain of rabies virus is required to lethality for adult mice. A cluster region of amino acid substitutions exists at the positions 164 to 303 on the glycopr...
Makoto Sugiyama, Kazuo Goto, Hiroko Uemukai, Yoshio Mori, Naoto Ito, Nobuyuki Minamoto
Journal of Veterinary Medical Science   66(4) 461-463   2004
To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 ...
Makoto Sugiyama, Naoto Ito, Nobuyuki Minamoto
Journal of Veterinary Medical Science   65(10) 1063-1068   Oct 2003
A sensitive and specific in situ amplification technique is needed to elucidate the dynamics of rabies virus in the body during the long incubation period after infection. To overcome the disadvantage of using the traditional reverse transcription...
Mohammed Ali Borgan, Yoshio Mori, Naoto Ito, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   47 661-668   2003
In order to analyze the antigenic structure of nonstructural protein (NSP) 4 of group A avian rotavirus strain PO-13, 25 monoclonal antibodies (MAbs) against NSP4 expressed in Escherichia coli were produced. All MAbs reacted with NSP4 on Western b...
Naoto Ito, Mutsuyo Takayama-Ito, Kentaro Yamada, Junji Hosokawa, Makoto Sugiyama, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   47 613-617   2003
To improve efficiency of recovery of rabies virus from cloned cDNA, we established a BHK cell clone that stably expresses T7 RNA polymerase, which we named BHK/T7-9. We also constructed new helper plasmids for expression of nucleoprotein and RNA p...
Yoshio Mori, Mohammed Ali Borgan, Mutsuyo Takayama, Naoto Ito, Makoto Sugiyama, Nobuyuki Minamoto
Virology   316(1) 126-134   2003
We previously demonstrated that a pigeon rotavirus, PO-13, but not turkey strains Ty-3 and Ty-1 and a chicken strain, Ch-1, induced diarrhea in heterologous suckling mice. In this study, it was suggested that these avirulent strains, but not PO-13...
Koji Tsuchida, T. Saigo, S. Tsujita, K. Takeuchi, N. Ito, M. Sugiyama
Molecular Ecology Notes   3(3) 384-386   2003
We isolated 13 microsatellite markers for the Japanese paper wasp Polistes chinensis antennalis. Null alleles were ruled out in the loci because we could detect polymerase chain reaction products for 578 haploid males. Our results indicate that th...
Heru Susetya, Makoto Sugiyama, Akio Inagaki, Naoto Ito, Kanisak Oraveerakul, Nimit Traiwanatham, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   47(9) 653-659   2003
We sequenced 512 nucleotides in two variable regions of the N gene of 23 rabies isolates from the northeastern part of Thailand by direct sequencing of PCR-amplified products. The sequencing data revealed two new lineages in these rabies isolates....
Yoshio Mori, Mohammed Ali Borgan, Naoto Ito, Makoto Sugiyama, Nobuyuki Minamoto
Virus Research   89(1) 145-151   2002
We determined the NSP4 sequences of turkey rotavirus strains Ty-1 and Ty-3 and a chicken rotavirus, strain Ch-1, and compared these sequences with those of a pigeon rotavirus, strain PO-13, and mammalian rotaviruses. The turkey strains and PO-13 w...
Makoto Sugiyama, Naoto Ito, Nobuyuki Minamoto, Seiichi Tanaka
Journal of Virology   76(4) 1691-1696   2002
The immunodominant epitopes on the hemagglutinin protein of rinderpest virus (RPV-H) were determined by analyzing selected monoclonal antibody (MAb)-resistant mutants and estimating the level of antibody against each epitope in five RPV-infected r...
Yoshio Mori, Mohammed Ali Borgan, Naoto Ito, Makoto Sugiyama, Nobuyuki Minamoto
Journal of Virology   76(11) 5829-5834   2002
Avian rotavirus NSP4 glycoproteins expressed in Escherichia coli acted as enterotoxins in suckling mice, as did mammalian rotavirus NSP4 glycoproteins, despite great differences in the amino acid sequences. The enterotoxin domain of PO-13 NSP4 exi...
N. Ito, M. Takayama, K. Yamada, M. Sugiyama, N. Minamoto
Journal of Virology   75(19) 9121-9128   2001
In order to identify the viral gene related to the pathogenicity of rabies virus, we tried to establish a reverse genetics system of the attenuated RC-HL strain, which causes nonlethal infection in adult mice after intracerebral inoculation. A ful...
H. Ito, M. Sugiyama, K. Masubuchi, Y. Mori, N. Minamoto
Virus Research   75(2) 123-138   2001
The nucleotide sequences encoding four structural proteins (VP1-4) and six nonstructural proteins (NSP1-6) of avian rotavirus PO-13 were determined. Based on the results of earlier sequencing studies [Ito et al., 1995, Sequence analysis of cDNA fo...
Yoshio Mori, Makoto Sugiyama, Mutsuyo Takayama, Yasuro Atoji, Toshiaki Masegi, Nobuyuki Minamoto
Virology   288(1) 63-70   2001
It has been suggested that group A avian rotaviruses can be transmitted to mammals, but there is no direct evidence that such viruses induce disease in mammals. Suckling mice were orally inoculated with two avian rotaviruses. A pigeon rotavirus, P...
N. Ito, M. Kakemizu, K. A. Ito, A. Yamamoto, Y. Yoshida, M. Sugiyama, N. Minamoto
Microbiology and Immunology   45(1) 51-58   2001
In order to establish the molecular basis of the pathogenicity of the attenuated RC-HL strain of rabies virus used for the production of animal vaccine in Japan, the complete genome sequence of this strain was determined and compared with that of ...
Hideo Goto, Hideo Goto, Nobuyuki Minamoto, Hiroshi Ito, Hiroshi Ito, Naoto Ito, Makoto Sugiyama, Toshio Kinjo, Akihiko Kawai
Journal of General Virology   81(1) 119-127   2000
Linear epitopes on the rabies virus nucleoprotein (N) recognized by six MAbs raised against antigenic sites I (MAbs 6-4, 12-2 and 13-27) and IV (MAbs 6-9, 7-12 and 8-1) were investigated. Based on our previous studies on sites I and IV, 24 consecu...
Naoto Ito, Makoto Sugiyama, Kanisak Oraveerakul, Prapruddee Piyaviriyakul, Boonlert Lumlertdacha, Yoko T. Arai, Yutaka Tamura, Yoshio Mori, Nobuyuki Minamoto, Nobuyuki Minamoto
Microbiology and Immunology   43(6) 551-559   Jan 1999
For the purpose of making clear the dynamics of rabies viruses that are prevalent among dogs in Asia, especially Thailand, nucleoprotein (N) genes of isolates derived from Thailand were partially sequenced, and a phylogenetic analysis was performe...
Ting Rong Luo, Nobuyuki Minamoto, Nobuyuki Minamoto, Miyuki Hishida, Keiko Yamamoto, Toru Fujise, Shinya Hiraga, Naoto Ito, Makoto Sugiyama, Toshio Kinjo
Microbiology and Immunology   42(3) 187-193   1998
Thirty-five monoclonal antibodies (MAbs) against glycoprotein (G protein) of the RC-HL strain of the rabies virus have been established. Using these MAbs, two antigenic sites (I and II) were delineated on the G protein of the RC-HL strain in a com...
Makoto Sugiyama, Rieko Yoshiki, Yuko Tatsuno, Shinya Hiraga, Osamu Itoh, Koichiro Gamoh, Nobuyuki Minamoto, Nobuyuki Minamoto
Clinical and Diagnostic Laboratory Immunology   4 727-730   1997
A competitive enzyme-linked immunosorbent assay (c-ELISA) was developed as an alternative to the viral neutralization (VN) test for rapid and simple detection of antibodies to rabies virus. The competitor antibody in the c-ELISA was a biotinylated...
Hiroshi Ito, Nobuyuki Minamoto, Shinya Hiraga, Makoto Sugiyama
Virus Research   47(1) 79-83   1997
cDNAs corresponding to the VP6 gene of the turkey rotavirus strains Ty-1 and Ty-3, and the chicken rotavirus strain Ch-1, were cloned and sequenced. The nucleotide and deduced amino acid sequence homology in the coding region of the VP6 gene in av...
Ting Rong Luo, Nobuyuki Minamoto, Hiroshi Ito, Hideo Goto, Shinya Hiraga, Naoto Ito, Makoto Sugiyama, Toshio Kinjo
Virus Research   51(1) 35-41   1997
We have established a hybridoma producing monoclonal antibody (MAb) against a linear epitope of glycoprotein (G protein) of the RC-HL strain of rabies virus. This MAb15-13 showed almost the same neutralizing activity to all of five rabies fixed st...
Anthony C. Woodman, Makoto Sugiyama, Kazuhiro Yoshida, Takashi Sugino, Annelies Borgya, Steven Goodison, Yasuhiro Matsumura, David Tarin, David Tarin
American Journal of Pathology   149 1519-1530   1996
Many studies have now demonstrated disorganized overexpression of the CD44 gene in various types of human malignant tumors, and this abnormality has emerged as an interesting candidate marker for early cancer diagnosis. The purpose of this work wa...
Makoto Sugiyama, Nobuyuki Minamoto, Toshio Kinjo, Hiroshi Ito, Kazuya Yamanouchi
Journal of Veterinary Medical Science   58(8) 769-771   1996
The sequence of the hemagglutinin (HA) gene of the LA strain of rinderpest virus (RPV) has been determined by the direct sequencing method. The amino acid sequence of the protein which it encodes displays conservation of its structural determinant...
H. Ito, N. Minamoto, N. Minamoto, H. Goto, T. R. Luo, M. Sugiyama, T. Kinjo
Archives of Virology   141(11) 2129-2138   1996
The cDNA encoding the VP6 gene of avian rotavirus PO-13 strain was inserted into the bacterial expression vector pET-3a. Upon isopropyl-1-thio- β-D-galactoside induction, the E. coli BL21 (DE3) harboring the vector containing cDNA of the VP6 gene ...
Ito Hiroshi, Nobuyuki Minamoto, Hideo Goto, Luo Ting Rong, Makoto Sugiyama, Toshio Kinjo
Veterinary Microbiology   49(3-4) 257-265   1996
A gene encoding the major inner capsid protein, VP6, of avian rotavirus was inserted into the eukaryotic expression vector pAX-91 under the control of the SR α promoter and was expressed at a high level in simian COS7 cells. The expressed VP6 was ...
M. Sugiyama, A. Woodman, T. Sugino, S. Crowley, K. Ho, J. Smith, Y. Matsumura, D. Tarin
Journal of Clinical Pathology - Clinical Molecular Pathology   48    Jan 1995
Aims - To investigate the expression of CD44 proteins in exfoliated urothelial cells and in tumour tissues from bladder cancer patients. A further objective was to evaluate the diagnostic potential of the changes observed in the expression of thes...
A. Borgya, A. Woodman, M. Sugiyama, F. Donie, E. Kopetzki, Y. Matsumura, D. Tarin
Journal of Clinical Pathology - Clinical Molecular Pathology   48 M241-M250   Jan 1995
Aims - Exon 7 of the human CD44 gene is overexpressed in many commonly occurring carcinomas. The aim of the study was to explore the diagnostic and therapeutic potential of this frequent abnormality. Methods - A new monoclonal antibody (mAb, M-23....
Unusual retention of introns in CD44 gene transcripts in bladder cancer provides new diagnostic and clinical oncological opportunites.
Journal of Pathology   177 11-20   1995
H. Ito, N. Minamoto, I. Sasaki, H. Goto, M. Sugiyama, T. Kinjo, S. Sugita
Archives of Virology   140(3) 605-612   1995
cDNA corresponding to the genomic segment 6 of avian rotavirus strain PO-13, which has group A common and subgroup I antigens, but does not hybridize in Northern blots with RNA probes from group A mammalian rotaviruses, was cloned and sequenced. W...
H. Goto, N. Minamoto, H. Ito, T. R. Luo, M. Sugiyama, T. Kinjo, A. Kawai
Archives of Virology   140(6) 1061-1074   1995
Investigations were performed to delineate the antigenic sites I and IV of rabies virus nucleoprotein (N), the former of which is well conserved among the rabies and rabies-related viruses. The N cDNA of the RC-HL strain was inserted into an expre...
Hideo Goto, Nobuyuki Minamoto, Hiroshi Ito, Makoto Sugiyama, Toshio Kinjo, Kazuaki Mannen, Kumato Mifune, Akihiko Kawai
Virus Genes   8 91-97   Jan 1994
By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC·HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA clones were obtained by Southern blot hy...
Y. Atoji, H. Watanabe, N. Nimamoto, M. Sugiyama, Y. Yamamoto, Y. Suzuki
European Journal of Histochemistry   38 65-72   Jan 1994
N. Minamoto, H. Tanaka, M. Hishida, H. Goto, H. Ito, S. Naruse, K. Yamamoto, M. Sugiyama, T. Kinjo, K. Mannen, K. Mifune
Microbiology and Immunology   38(6) 449-455   Jan 1994
A set of 29 monoclonal antibodies (MAbs) specific for the rabies virus nucleoprotein (N protein) was prepared and used to analyze the topography of antigenic sites. At least four partially overlapping antigenic sites were delineated on the N prote...
H. Ito, N. Minamoto, T. Watanabe, H. Goto, L. T. Rong, M. Sugiyama, T. Kinjo, K. Mannen, K. Mifune, T. Konobe, I. Yoshida, A. Takamizawa
Microbiology and Immunology   38(6) 479-482   Jan 1994
Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This resul...
H. Kamata, S. Ohkubo, M. Sugiyama, Y. Matsuura, Y. Kamata, K. Tsukiyama-Kohara, K. Imaoka, C. Kai, Y. Yoshikawa, K. Yamanouchi
Journal of Virological Methods   43(2) 159-165   Jan 1993
The rinderpest (RV) nucleocapsid (NP) gene segment was inserted into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV) adjacent to the polyhedrin promoter. The expression of NP protein in Sf9 cells was confirmed by indirect i...
N. Minamoto, O. Sugimoto, M. Yokota, M. Tomita, H. Goto, M. Sugiyama, T. Kinjo
Archives of Virology   131(3/4) 293-305   1993
Monoclonal antibodies (MAbs) were prepared to analyze antigens on the major inner capsid protein, VP 6 of avian group A rotavirus. Based on the results of a competitive binding assay using 15 MAbs directed against VP 6 of the PO-13 rotavirus strai...
T. Suzuki, N. Minamoto, M. Sugiyama, T. Kinjo, Y. Suzuki, M. Sugimura, Y. Atoji
Journal of wildlife diseases   29 384-389   1993
An epizootic suspected to be caused by parapoxviruses occurred in winter, 1984-85, among wild Japanese serows (Capricornis crispus) with nodular or papular lesions in Gifu Prefecture, Japan. Virus isolations were attempted on 30 animals using bovi...
T. Kinjo, N. Minamoto, M. Sugiyama, Y. Sugiyama
The Journal of veterinary medical science / the Japanese Society of Veterinary Science   54(5) 821-827   1992
The fecal Escherichia coli isolated from wild Japanese serows living in mountainous areas away from humans and those from captive serows kept in human areas were examined for antimicrobial resistance and the possession of transferable R plasmids. ...
M. Sugiyama, N. Minamoto, T. Kinjo, N. Hirayama, K. Asano, K. Y. Tsukiyama-Kohara Yoshikawa, K. Yamanouchi
Journal of General Virology   72(8) 1863-1869   Mar 1991
A total of 24 monoclonal antibodies (MAbs) against the haemagglutinin (H) and the fusion protein (F) of rinderpest virus (RPV) were used to characterize their antigenic structure and biological properties, and to analyse natural variation in the e...
Hiroshi Kamata, Hiroshi Kamata, Kyoko Tsukiyama, Makoto Sugiyama, Yasue Kamata, Yasuhiro Yoshikawa, Kazuya Yamanouchi
Virus Genes   5, 5-15 5-15   1991
The full-length cDNA corresponding to the mRNA encoding the nucleocapsid protein (NP) of rinderpest virus (RV) was cloned and its complete nucleotide sequence was determined. The gene of RV-NP was composed of 1683 nucleotides and contained a singl...
N. Hirayama, M. Senda, N. Nakashima, M. Takagi, M. Sugiyama, Y. K. Yoshikawa Yamanouchi
Journal of General Virology   72(11) 2827-2830   1991
Monoclonal antibodies (MAbs) against the haemagglutinin (H), fusion protein (F) and nucleoprotein of canine distemper virus (CDV) were examined for their ability to protect mice against lethal CDV infection. One MAb against H and two of six MAbs a...
T. Asai, T. Kinjo, N. Minamoto, M. Sugiyama, N. Matsubayashi, I. Narama
The Journal of veterinary medical science / the Japanese Society of Veterinary Science   53(4) 553-559   1991
The prevalence of antibodies against 5 zoonosis agents was determined in serum samples of 443 breeding monkeys. Of the monkeys, 296 were bred or kept for a long time at R institute, and the remaining 147 were newly imported from the Philippines an...
N. Minamoto, O. Nakagomi, M. Sugiyama, T. Kinjo
Research in Virology   142(4) 271-275   1991
Pigeon rotavirus strain PO-13, which was recently shown to be neutralized by a hyperimmune serum to the prototype serotype 7 virus Ch-2, showed a one-way neutralization cross with turkey rotavirus Ty-1. When its genome was compared by RNA-RNA hybr...
F. Kobune, H. Sakata, M. Sugiyama, A. Sugiura
Journal of General Virology   72(3) 687-692   1991
We reported earlier that B95a, an Epstein-Barr virus-transformed marmoset B lymphoblastoid cell line, is more susceptible to infection with measles virus than other cells. The cell line also was found to be susceptible to infection with the lapini...
M. Sugiyama, N. Hirayama, H. Sasaki, T. Sugimura, N. Minamoto, T. Kinjo
Research in Veterinary Science   46, 283-285 283-285   Mar 1989   [Refereed]
Twelve monoclonal antibodies against ibaraki virus (IbV) were established and preliminarily characterised by indirect immunoperoxidase (IIP), haemagglutination inhibition (HI) and neutralisation (NT) tests. Five antibodies reacted in the IIP test ...
M. Sugiyama, N. Minamoto, T. Kinjo, N. Hirayama, H. Sasaki, Y. Yoshikawa, K. Yamanouchi
Journal of General Virology   70 2605-2613   1989
Yasuhiro Yoshikawa, Fumiko Ochikubo, Yutaka Matsubara, Hiroshi Tsuruoka, Mieko Ishii, Kinji Shirota, Yasuo Nomura, Makoto Sugiyama, Kazuya Yamanouchi
Veterinary Microbiology   20, 193-205 193-205   1989
A case of encephalitis in a Japanese monkey (Macaca fuscata) was examined histopathologically and serologically. The animal had brain lesions consisting of perivascular cuffs, malacia, inclusion bodies and giant cells. Monoclonal antibody to the n...
Kyoko Tsukiyama, Makoto Sugiyama, Yasuhiro Yoshikawa, Kazuya Yamanouchi
Virology   160(1) 48-54   1987
We cloned the full-length cDNAs corresponding to the mRNA for the hemagglutinin[dH) protein of rinderpest virus (RV) and determined the nucleotide sequence of RV-H. The gene of RV-H was composed of 1952 nucleotides and contained a single large ope...
O. Itoh, M. Sugiyama, S. Nakamura, H. Sasaki
Microbiology and Immunology   28(10) 1163-1167   1984
Sugiyama, M., Minamoto, N., Kinjo, T. and Hashimoto, A
Japanese Journal of Veterinary Science   46(5) 767-771   1984
Drug resistance and R plasmids in Escherichia coli strains isolated from feces of imported pet birds.
Kinjo, T., Minamoto, N., Sugiyama, M., Sugiyama, Y. and Hirai, K.
Res. Bull. Fac. Agr. Gifu Univ.   45 179-186   1981   [Refereed]

Misc

 
Difference in ability to cause interference of homologous strains of bovine viral diarrheamucosal disease virus.
Sugiyama, M., Itoh, O. and Sasaki, H.
Annual Report of National Veterinary Assay Laboratory   21 11-17   1984
Makoto Sugiyama, Naoto Ito, Naoto Ito
Comparative Immunology, Microbiology and Infectious Diseases   30(5-6) 273-286
Rabies is an enzootic viral disease widespread throughout the world. Although it is a vaccine-preventable disease, the annual number of human deaths caused by rabies is estimated to be 32,000 in Asia. Phylogenetic analysis based on sequence data o...
Naoto Ito, Makoto Sugiyama
Virus   57(2) 191-198   2007
Rabies virus causes lethal neurological symptoms in humans and animals. Rabies epidemics have continued to occur throughout the world, despite the fact that rabies can be effectively prevented by vaccination. The development of inexpensive and saf...
Ito, N. and Moseley, G.W. and Sugiyama, M.
J. Vet. Med. Sci.   78(7) 1089-1098   2016

Research Grants & Projects

 
Studies on ecology and pathogenesis of viral zoonotic agent
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 1997 - 1999    Investigator(s): Makoto SUGIYAMA
We had attempted to develop a competitive enzyme-linked immunosorbent assay (c-ELISA) for rapid and simple detection of a specific antibody to each morbillivirus. Twenty one monoclonal antibodies to the nucleoprotein (NP) and hemagglutinin protein...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2000 - 2002    Investigator(s): Makoto SUGIYAMA
The receptor(s) for a pigeon rotavirus, PO-13, on MA104 cells and Vero cells derived from monkey, and CER cells derived from hamster was/were stained by using its purified VP8 protein expressed in bacteria. The receptor for PO-13 strain was expres...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2003 - 2005    Investigator(s): Makoto SUGIYAMA
The latex agglutination (LA) tests for detection of P genotype-specific antibody to Group A rotavirus were established. The antibodies to P[2] and [3] rotaviruses, which were epidemiologically relevant in some animal species, were detected by the ...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2006 - 2007    Investigator(s): Makoto SUGIYAMA
We investigated the prevalence and infection of group A rotaviruses in the healthy calves and wild boars which were thought that various rotaviruses are contagious at high frequency by our previous seroepidemiological study. One of 135 samples of ...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2011 - 2013    Investigator(s): Makoto SUGIYAMA
The aim of this study was to generate a replication-defective (M gene-deficient) rabies virus strain by gene manipulation and to examine the potency of the strain as a rabies vaccine. Our preliminary data strongly suggested that the M gene-deficie...
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2011 - 2013
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2013 - 2015
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2013 - 2015
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2014 - 2016