In this paper, a modified seed-mediated growth approach to produce gold nanoparticles through HAuCl 4 chemical reduction in water fabricated by growth process on indium tin oxide (ITO) was proposed. Particular attention was devoted to exploring the seeding and growth number cycle process in the formation of Au nanoparticles on the ITO surface. In agreement with the assumed analytical model, we have found that the absorbance maximum intensity λmax depends substantially on the metal nanoparticles' sizes, shape and density on the ITO surface. The deposited nanoparticles' synthesized parameters were evaluated by the surface images obtained using field emission scanning electron microscopy (FE-SEM), UV-Vis spectroscopy and electrochemical measurements. The results show that the electrochemical responses of the as-prepared sample were significantly improved, in particular for the 2-cycle seeded particles followed by one-cycle growth.