2004年1月
Self-organized glycoclusters along DNA: Effect of the spatial arrangement of galactoside residues on cooperative lectin recognition
CHEMISTRY-A EUROPEAN JOURNAL
- ,
- ,
- ,
- ,
- 巻
- 10
- 号
- 2
- 開始ページ
- 352
- 終了ページ
- 359
- 記述言語
- 英語
- 掲載種別
- 書評論文,書評,文献紹介等
- DOI
- 10.1002/chem.200305465
- 出版者・発行元
- WILEY-V C H VERLAG GMBH
We describe herein the relationship between the spatial arrangement of self-organized galactose clusters and lectin recognition. beta-Galactose-modified deoxyuridine phosphoramidite was synthesized and applied to solid-phase synthesis to provide 18-, 20-, and 22-mers of site-specifically galactosylated oligodeoxynucleotides (Gal-ODNs). These Gal-ODNs were self-organized through hybridization with the corresponding 18-, 20-, and 22-mers of half-sliding complementary ODNs (hsc-ODNs) to give periodic galactoside clusters. The self-organization of ODNs was confirmed by size exclusion chromatography and gel electrophoresis. The binding of the Gal-clusters to the FITC-labeled RCA(120) lectin was analyzed by monitoring the change in fluorescence intensity. The assembly of 20-mer Gal-ODN with the 20-mer hsc-ODN was strongly and co-operatively recognized by the lectin. The 18-mer assembly was bound more weakly and less cooperatively, and the 22-mer assembly was minimally bound to the lectin. RCA(120) lectin recognized not only the density of galactoside residues, but also the spatial arrangement. The size of the Gal cluster was estimated from the association constant of Gal-ODN with hsc-ODN. The relationship between lectin-recognition and Gal-cluster size is also discussed.
- リンク情報
-
- DOI
- https://doi.org/10.1002/chem.200305465
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016437791
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/14735503
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000188712200004&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1002/chem.200305465
- ISSN : 0947-6539
- eISSN : 1521-3765
- CiNii Articles ID : 80016437791
- PubMed ID : 14735503
- Web of Science ID : WOS:000188712200004