2004年5月
Surface plasmon resonance study of carbohydrate-carbohydrate interaction between various gangliosides and Gg3-carrying polystyrene
BIOMACROMOLECULES
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- ,
- ,
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- 巻
- 5
- 号
- 3
- 開始ページ
- 937
- 終了ページ
- 941
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1021/bm034508g
- 出版者・発行元
- AMER CHEMICAL SOC
Carbohydrate-carbohydrate interactions between Gg3 trisaccharide-carrying polystyrene (PN(Gg3)) and monolayers of several glycosphingolipids (GSLs) were quantitatively investigated by Surface plasmon resonance techniques. PN(Gg3) was adsorbed onto a GM3 monolayer strongly and specifically with an apparent affinity constant of K-a = 2.5 x 10(6) M-1, and the apparent affinity constants onto GSLs decreased ill the following order: GM3 > LacCer > (KDN)GM3 approximate to GlcCer > GM2 approximate to GD3 approximate to GM4 > GM1 approximate to 2.6-isoGM3 > ceramide. These results suggest that PN(G-3) recognizes not only some specified portions of GM3 but also the trisaccharide as a whole. On the other hand, PN(Lac) and PN(Cel) were bound to GSLs less strongly (K-a approximate to 10(4) M-1) and less selectively. The kinetic analysis revealed that the selectivity ill the adsorption of PN(Gg3) onto the GM3 monolayer is dominated by the faster adsorption rate.
- リンク情報
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- DOI
- https://doi.org/10.1021/bm034508g
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016745437
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15132684
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221315200042&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1021/bm034508g
- ISSN : 1525-7797
- eISSN : 1526-4602
- CiNii Articles ID : 80016745437
- PubMed ID : 15132684
- Web of Science ID : WOS:000221315200042