2018年4月1日
Induction of somatic mutations by low-dose X-rays: The challenge in recognizing radiation-induced events
Journal of Radiation Research
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 59
- 号
- supple 2
- 開始ページ
- ii11
- 終了ページ
- ii17
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jrr/rrx053
- 出版者・発行元
- Oxford University Press
It is difficult to distinguish radiation-induced events from spontaneous events during induction of stochastic effects, especially in the case of low-dose or low-dose-rate exposures. By using a hypersensitive system for detecting somatic mutations at the HPRT1 locus, we investigated the frequency and spectrum of mutations induced by low-dose X-rays. The mutant frequencies induced by doses of >
0.15 Gy were statistically significant when compared with the spontaneous frequency, and a clear dose dependency was also observed for mutant frequencies at doses of >
0.15 Gy. In contrast, mutant frequencies at doses of <
0.1 Gy occurred at non-significant levels. The mutation spectrum in HPRT-deficient mutants revealed that the type of mutations induced by low-dose exposures was similar to that seen in spontaneous mutants. An apparent change in mutation type was observed for mutants induced by doses of >
0.2 Gy. Our observations suggest that there could be a critical dose for mutation induction at between 0.1 Gy and 0.2 Gy, where mutagenic events are induced by multiple DNA double-strand breaks (DSBs). These observations also suggest that low-dose radiation delivered at doses of <
0.1 Gy may not result in DSB-induced mutations but may enhance spontaneous mutagenesis events.
0.15 Gy were statistically significant when compared with the spontaneous frequency, and a clear dose dependency was also observed for mutant frequencies at doses of >
0.15 Gy. In contrast, mutant frequencies at doses of <
0.1 Gy occurred at non-significant levels. The mutation spectrum in HPRT-deficient mutants revealed that the type of mutations induced by low-dose exposures was similar to that seen in spontaneous mutants. An apparent change in mutation type was observed for mutants induced by doses of >
0.2 Gy. Our observations suggest that there could be a critical dose for mutation induction at between 0.1 Gy and 0.2 Gy, where mutagenic events are induced by multiple DNA double-strand breaks (DSBs). These observations also suggest that low-dose radiation delivered at doses of <
0.1 Gy may not result in DSB-induced mutations but may enhance spontaneous mutagenesis events.
- リンク情報
- ID情報
-
- DOI : 10.1093/jrr/rrx053
- ISSN : 1349-9157
- ISSN : 0449-3060
- SCOPUS ID : 85041464778