2009年9月
Development of a conjugated gadolinium and cisplatin-gelatin possessing properties as an intravascular contrast agent for MR imaging
EUROPEAN JOURNAL OF RADIOLOGY
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- 巻
- 71
- 号
- 3
- 開始ページ
- 570
- 終了ページ
- 575
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ejrad.2008.04.009
- 出版者・発行元
- ELSEVIER IRELAND LTD
Purpose: The purpose of this study is to create a Gd-DTPA-Gel-Cis compound, which made from gadolinum (Gd), diethylenetriaminepentaacetic acid (DTPA)-dianhydride, cis-diamminedichloroplatinum (Cis) and bovine gelatin (Gel), that makes it possible to visualize Cis as intravascular agent under magnetic resonance imaging (MRI).
Materials and methods: The amount of DTPA, Gd, and Cis were titrated to determine the new compound's conjugation ratio with gelatin. Considering these functions, Gd-DTPA-Gel-Cis was synthesized, and its stability in bovine serum was evaluated. In addition, the signal intensity of the diluted sample was measured under 1.5 Tesla MRI.
Results: The synthesized 10 mg/ml of Gd-DTPA-Gel-Cis contained 42.84 mu g/ml of Gd and 1.53 mu g/ml of platinum. Gd-DTPA-Gel-Cis (100 mg/10 ml) enclosed into the cellulose dialysis tubing was placed in 90 ml of bovine serum and shaken reciprocally at 72 stroke/min at 37 degrees C. Partial release of free Pt was shown at 6 and 24 h, but no release of Gd occurred for a 24-h period. And high stability of Gd conjugated to DTPA-Gel-Cis. This result suggests possible anti-tumor effectiveness and high stability of Gd conjugated to DTPA-Gel-Cis. The diluted sample presented high signal intensity under 1.5 Tesla MRI.
Conclusion: Gd-DTPA-Gel-Cis has been developed successfully and we have proven its stability and contrast ability in MRI. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Materials and methods: The amount of DTPA, Gd, and Cis were titrated to determine the new compound's conjugation ratio with gelatin. Considering these functions, Gd-DTPA-Gel-Cis was synthesized, and its stability in bovine serum was evaluated. In addition, the signal intensity of the diluted sample was measured under 1.5 Tesla MRI.
Results: The synthesized 10 mg/ml of Gd-DTPA-Gel-Cis contained 42.84 mu g/ml of Gd and 1.53 mu g/ml of platinum. Gd-DTPA-Gel-Cis (100 mg/10 ml) enclosed into the cellulose dialysis tubing was placed in 90 ml of bovine serum and shaken reciprocally at 72 stroke/min at 37 degrees C. Partial release of free Pt was shown at 6 and 24 h, but no release of Gd occurred for a 24-h period. And high stability of Gd conjugated to DTPA-Gel-Cis. This result suggests possible anti-tumor effectiveness and high stability of Gd conjugated to DTPA-Gel-Cis. The diluted sample presented high signal intensity under 1.5 Tesla MRI.
Conclusion: Gd-DTPA-Gel-Cis has been developed successfully and we have proven its stability and contrast ability in MRI. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.ejrad.2008.04.009
- ISSN : 0720-048X
- Web of Science ID : WOS:000270644400028