2010年
Augmented Anti-tumor Effect of Dendritic Cells Genetically Engineered by Interleukin-12 Plasmid DNA
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
- ,
- ,
- 巻
- 21
- 号
- 5
- 開始ページ
- 659
- 終了ページ
- 675
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1163/156856209X434674
- 出版者・発行元
- VSP BV
The objective of this study was to genetically engineer dendritic cells (DC) for biological activation and evaluate their anti-tumor activity in a tumor-bearing mouse model. Mouse DC were incubated on the surface of culture dishes which had been coated with the complexes of a cationized dextran and luciferase plasmid DNA complexes plus a cell adhesion protein, Pronectin (R), for gene transfection (reverse transfection). When compared with the conventional transfection where DC were transfected in the medium containing the complexes, the level of gene expression by the reverse method was significantly higher and the time period of gene expression was prolonged. Following the reverse transfection of DC by a plasmid DNA of mouse interleukin-12 (mIL-12) complexed with the cationized dextran, the mIL-12 protein was secreted at higher amounts for a longer time period. When injected intratumorally into mice carrying a mass of B16 tumor cells, the DC genetically activated showed significant anti-tumor activity. (C) Koninklijke Brill NV, Leiden, 2010
- リンク情報
- ID情報
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- DOI : 10.1163/156856209X434674
- ISSN : 0920-5063
- Web of Science ID : WOS:000275981700009