2009年10月
Investigation Using an HER-2/neu Transgenic Mouse Model of a Newly Developed MR Contrast Agent With the Effect of an Antitumor Drug
JOURNAL OF MAGNETIC RESONANCE IMAGING
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- 巻
- 30
- 号
- 4
- 開始ページ
- 907
- 終了ページ
- 910
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jmri.21911
- 出版者・発行元
- JOHN WILEY & SONS INC
Purpose: To assess whether Gd-DTPA-Gel-Cis, a conjugate of gadolinium (Gd), cis diamminedichlotoplatinum (Cis), diethylenetriaminepentaacetic acid. (DTPA)-dianhydride, and bovine gelatin (Gel) can be used as an intravascular contrast agent at MRI and as an antitumor cell proliferation agent in vitro.
Materials and Methods: We injected Gd-DTPA-Gel-Cis (200 mg/mL) into the caudal vein of female HER-2/neu transgenic mice with spontaneous mammary tumors. The tumor signal intensity was measured with a 0.3 Tesla MRI scanner. HER-2/neu-expressing NT cells were treated with Gd-DTPA-Gel-Cis (5 mu M cisplatin, 200 mg/mL Gel), Cis alone (5 mu M cisplatin), or Gel alone (200 mg/mL gelatin). Differences of P < 0.05 were considered to be statistically significant.
Results: On T1-weighted MRI scans of mice injected with Gd-DTPA-Gel-Cis we observed a 23% increase in signal intensity. The survival rates of cells exposed to Gd-DTPA-Gel-Cis or Cis were 70.9% and 58.3%, respectively, of the survival rates observed after treatment with Gel alone. Gd-DTPA-Gel-Cis showed significant toxicity (P < 0.05).
Conclusion: Gd-DTPA-Gel-Cis shows promise for use as an MRI contrast medium and as an antitumor agent.
Materials and Methods: We injected Gd-DTPA-Gel-Cis (200 mg/mL) into the caudal vein of female HER-2/neu transgenic mice with spontaneous mammary tumors. The tumor signal intensity was measured with a 0.3 Tesla MRI scanner. HER-2/neu-expressing NT cells were treated with Gd-DTPA-Gel-Cis (5 mu M cisplatin, 200 mg/mL Gel), Cis alone (5 mu M cisplatin), or Gel alone (200 mg/mL gelatin). Differences of P < 0.05 were considered to be statistically significant.
Results: On T1-weighted MRI scans of mice injected with Gd-DTPA-Gel-Cis we observed a 23% increase in signal intensity. The survival rates of cells exposed to Gd-DTPA-Gel-Cis or Cis were 70.9% and 58.3%, respectively, of the survival rates observed after treatment with Gel alone. Gd-DTPA-Gel-Cis showed significant toxicity (P < 0.05).
Conclusion: Gd-DTPA-Gel-Cis shows promise for use as an MRI contrast medium and as an antitumor agent.
- リンク情報
- ID情報
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- DOI : 10.1002/jmri.21911
- ISSN : 1053-1807
- Web of Science ID : WOS:000270522900030