論文

査読有り
2009年7月

Practical Induction System for Dopamine-Producing Cells from Bone Marrow Stromal Cells Using Spermine-Pullulan-Mediated Reverse Transfection Method

TISSUE ENGINEERING PART A
  • Kentaro Nagane
  • ,
  • Masaaki Kitada
  • ,
  • Shohei Wakao
  • ,
  • Mari Dezawa
  • ,
  • Yasuhiko Tabata

15
7
開始ページ
1655
終了ページ
1665
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/ten.tea.2008.0453
出版者・発行元
MARY ANN LIEBERT INC

Introduction of various kinds of exogenous genes is an important step for control of differentiation in stem cell biology and regenerative medicine. However, some kinds of cells are vulnerable to manipulations such as gene delivery. In this context, a gene introduction method with higher efficiency and safety is required. Bone marrow stromal cells (BMSCs) offer possibilities for clinical application because of their potential for expandability and ability to be auto-transplanted. In this study, we established an efficient induction system of dopamine-producing neuronal cells from BMSCs in several species using the spermine-pullulan-mediated reverse transfection technique. In this system, introduced exogenous plasmid genes were successfully transcribed and expressed as proteins in the cytoplasm of BMSCs with the smallest number of cell death. Microtubule-associated protein 2 and anti-beta-tubulin class III(+) neurons were successfully delivered from human, monkey, and mouse BMSCs, and further treatment with trophic factors promoted differentiation of induced neuronal cells into dopamine-producing cells that were positive for tyrosine hydroxylase and secreted dopamine after high K(+) stimulation in high-performance liquid chromatography analysis. Our study indicates the availability of the reverse transfection method for the induction of dopamine-producing neuronal cells from BMSCs, which is expected to apply to cell-based therapy in Parkinson's disease.

リンク情報
DOI
https://doi.org/10.1089/ten.tea.2008.0453
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000267843700021&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/ten.tea.2008.0453
  • ISSN : 1937-3341
  • Web of Science ID : WOS:000267843700021

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