2009年8月
Effect of gelatin hydrogel incorporating fibroblast growth factor 2 on human meniscal cells in an organ culture model
KNEE
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- 巻
- 16
- 号
- 4
- 開始ページ
- 285
- 終了ページ
- 289
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.knee.2008.12.011
- 出版者・発行元
- ELSEVIER SCIENCE BV
Efforts to use growth factors to enhance the healing potential of the meniscus have been impeded because their half-lives are too short to maintain the biological activity. The thread was coated with gelatin hydrogel and fibroblast growth factor 2 (FGF 2) was biologically stabilized by incorporating in a gelatin hydrogel-coated thread. The purpose of this study is to investigate the effect of gelatin hydrogel-coated thread incorporating FGF 2 on human meniscal cells in an organ Culture. Twenty-five menisci were cut into small pieces, and selected pieces were sutured with gelatin hydrogel-coated thread incorporating FGF 2 (FGF(+) group) or physiologic saline (FGF(-) group), followed by organ Culture. The meniscal samples histologically evaluated 4, 7, and 14 days later. The cell density and the number of PCNA-positive cells for the FGF(+) group were higher than those of the FGF(-) group, while the number of TUNEL-positive cells was lower. These results Suggest that FGF 2 stimulates the proliferation of meniscal cells and inhibits meniscal cell death. Gelatin hydrogel-coated threads releasable FGF 2 may be useful to promote repairing of human meniscus. (C) 2008 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.knee.2008.12.011
- ISSN : 0968-0160
- Web of Science ID : WOS:000267776800011