論文

2005年6月

Osteogenic differentiation of mesenchymal stem cells in biodegradable sponges composed of gelatin and beta-tricalcium phosphate

BIOMATERIALS
  • Y Takahashi
  • ,
  • M Yamamoto
  • ,
  • Y Tabata

26
17
開始ページ
3587
終了ページ
3596
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2004.09.046
出版者・発行元
ELSEVIER SCI LTD

Biodegradable gelatin sponges incorporating various amounts of beta-tricalcium phosphate (betaTCP) (gelatin-betaTCP) were fabricated and the in vitro osteogenic differentiation of mesenchymal stem cells (MSC) isolated from the rat bone marrow in the sponges was investigated. The gelatin sponges incorporating betaTCP have an interconnected pore structure with the average size of 180-200 gm, irrespective of the betaTCP amount. The stiffness of the sponges became higher with an increase in the amount of betaTCP. When seeded into the sponges by an agitated method, MSC were homogeneously distributed throughout the sponge. The morphology of cells attached got more spreaded with the increased betaTCP amount. The rate of MSC proliferation depended on the betaTCP amount and culture method: the higher the betaTCP amount in the stirring culture, the higher the proliferation rate. The deformed extent of gelatin-betaTCP sponges was suppressed with the increased amount of betaTCP. When measured to evaluate the osteogenic differentiation of MSC, the alkaline phosphatase activity and osteocalcin content became maximum for the sponge with a betaTCP amount of 50 wt%, although both the values were significantly high in the stirring culture compared with those in the static culture. We concluded that the attachment, proliferation, and osteogenic differentiation of MSC were influenced by sponge composition of gelatin and betaTCP as the cell scaffold. (C) 2004 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2004.09.046
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000226968200024&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.biomaterials.2004.09.046
  • ISSN : 0142-9612
  • Web of Science ID : WOS:000226968200024

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