2003年4月
Bioartificial pancreas transplantation at prevascularized intermuscular space: Effect of angiogenesis induction on islet survival
PANCREAS
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- 巻
- 26
- 号
- 3
- 開始ページ
- 279
- 終了ページ
- 285
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- LIPPINCOTT WILLIAMS & WILKINS
Introduction: Bioartificial pancreas (BAP) transplantation offers a potential treatment of diabetes mellitus. The optimal site for BAP transplantation has not yet been established. Aim: To monitor the effect of induction of neovascularization at the intermuscular space on islet survival after allogenic transplantation of BAR Methodology: Angiogenesis was induced at the intermuscular space of diabetic Lewis rats by implanting a polyethylene terephthalate (PET) mesh bag, which enclosed a collagen sponge and biodegradable gelatin microspheres containing basic fibroblast growth factor. After confirmation of angiogenesis, BAP was prepared by mixing of 5% agarose with approximate to2,800 isolated rat (Sprague-Dawley) islets and transplanted into the prevascularized PET mesh bag. Results: Neovascularization was observed in and around the PET mesh bag within 10 days after implantation as confirmed by macroscopic and microscopic examinations. In the presence of a collagen sponge, new blood vessels penetrated into the PET mesh bag and formed a vascular bed. After transplantation, normoglycemia was achieved in the rats within 3 days and maintained for >35 days. The rats gradually gained body weight, and the results of intravenous glucose tolerance test showed normal patterns of blood glucose clearance 1 month after transplantation. Conclusion: It can be concluded that the prevascularized PET mesh bag enabled transplanted BAP to survive and maintain function, thus indicating a potential site for BAP transplantation.
- リンク情報
- ID情報
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- ISSN : 0885-3177
- Web of Science ID : WOS:000181905800012