2014年8月
Glutathione contributes to the efflux of selenium from hepatoma cells
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 78
- 号
- 8
- 開始ページ
- 1376
- 終了ページ
- 1380
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2014.918487
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Selenite is a selenium source for selenoprotein biosynthesis in mammalian cells. Although previous studies have suggested the involvement of glutathione (GSH) and/or thioredoxin reductase in selenite metabolism, intracellular selenite metabolism remains largely unknown. Here, we report that GSH depletion did not affect the amount of selenoprotein in Hepa 1-6 cells, suggesting that GSH does not play a central role in the reduction of selenite in selenoprotein biosynthesis. On the other hand, we found that GSH is involved in the efflux of low-molecular-weight selenium compounds from cells, presumably via the formation of selenodiglutathione. Moreover, selenite inhibited the efflux of a fluorescent bimane-GS conjugate that is mediated by ATP-dependent multidrug-resistant proteins, implying the existence of an active transporter for selenodiglutathione. This is the first report demonstrating that GSH plays a role in selenium excretion from cells by forming a GSH-conjugate, which may contribute to the distribution, detoxification, and homeostasis of selenium in the body.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2014.918487
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000340722500013