MISC

2002年6月

Preparation of high activity yeast whole cell bioctalysts by optimization of intracellular production of recombinant Rhizopus oryzae lipase

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
  • T Matsumoto
  • ,
  • S Takahashi
  • ,
  • M Ueda
  • ,
  • A Tanaka
  • ,
  • H Fukuda
  • ,
  • A Kondo

17
3-5
開始ページ
143
終了ページ
149
記述言語
英語
掲載種別
DOI
10.1016/S1381-1177(02)00021-8
出版者・発行元
ELSEVIER SCIENCE BV

Yeast whole cell biocatalysts, which intracellularly overproduced a recombinant lipase with a pro-sequence from Rhizopus oryzae IFO4697 (rProROL) were constructed, and the content of active lipase in Saccharomyces cerevisiae cells was maximized by optimizing the cultivation procedure. rProROL was overproduced intracellularly under the control of the 5'-upstream region of the isocitrate lyase gene of Candida tropicalis (UPR-ICL) as the inducible system and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter as the constitutive expression system. Enhancement of expression level of ProROL gene at the initial cultivation phase inhibited rProROL accumulation in yeast cells both in GAPDH promoter system by using high glucose concentration at 30 C and in UPR-ICL system by using non-fermentable carbon sources. The highest intracellular lipase activity of 350.6IU/l was obtained in the inducible UPR-ICL system with an initial glucose concentration of 0.5% at 30 C. To prepare the efficient whole cell biocatalyst by intracellular overproduction of lipase, utilization of inducible UPR-ICL and the optimization of cultivation conditions such as temperature, carbon source and its initial concentration are important. (C) 2002 Elsevier Science B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/S1381-1177(02)00021-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000176161400005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/S1381-1177(02)00021-8
  • ISSN : 1381-1177
  • Web of Science ID : WOS:000176161400005

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