1994年1月
CHARACTERIZATION OF THE CATALASE OF THE N-ALKANE-UTILIZING YEAST CANDIDA-TROPICALIS FUNCTIONALLY EXPRESSED IN SACCHAROMYCES-CEREVISIAE
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- ,
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- 巻
- 40
- 号
- 5
- 開始ページ
- 682
- 終了ページ
- 686
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/BF00173329
- 出版者・発行元
- SPRINGER VERLAG
Candida tropicalis catalase (CTC) genomic DNA was recombined on a plasmid with the galactose-inducible GAL7 promoter and expressed highly as a heme protein in Saccharomyces cerevisiae as host. The percentage of recombinant CTC (rCTC) in total extractable protein amounted to at least 25%. The rCTC was purified and characterized in terms of subunit mass, behavior in native polyacrylamide gel electrophoresis, absorption spectrum, amino-terminal amino acid sequence, peptide map, specific activity, and Michaelis constant (K-m) value for hydrogen peroxide. These properties were similar or identical to those of the purified enzyme from C. tropicalis (CTC). From these results, this system appears suitable for high expression of functional catalase protein having heme.
- リンク情報
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- DOI
- https://doi.org/10.1007/BF00173329
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902108042311291
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/7764426
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1994MZ71300017&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/BF00173329
- ISSN : 0175-7598
- J-Global ID : 200902108042311291
- PubMed ID : 7764426
- Web of Science ID : WOS:A1994MZ71300017