1996年10月
Individual expression of Candida tropicalis peroxisomal and mitochondrial carnitine acetyltransferase-encoding genes and subcellular localization of the products in Saccharomyces cerevisiae
JOURNAL OF BIOCHEMISTRY
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- 巻
- 120
- 号
- 4
- 開始ページ
- 731
- 終了ページ
- 735
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- JAPAN BIOCHEMICAL SOC
In an n-alkane-assimilating yeast, Candida tropicalis, carnitine acetyltransferase (CAT; EC 2.3.1.7) was localized in both peroxisomes and mitochondria. Both CATs were encoded by one gene, CT-CAT, although the initiation sites of translation were suggested to be different. In the present study, the genes corresponding ao the supposed C. tropicalis peroxisomal and mitochondrial CATs, which were truncated from the CT-CAT gene, were individually expressed in Saccharomyces cerevisiae, using the C. tropicalis isocitrate lyase promoter (UPR-ICL), which is inducible by oleic acid in concert with proliferation of peroxisomes in S. cerevisiae [Umemura, K., Atomi, H., Kanai, T., Teranishi, Y., Ueda, M., and Tanaka, A. (1995) Appl. Microbiol. Biotechnol. 43, 489-492]. The 71 kDa precursor of mitochondrial CAT, initiating at the first Met, was found to be processed to the mature size (66 kDa) in S. cerevisiae and immunoelectronmicroscopical observation revealed that this enzyme was localized in mitochondria. On the other hand, 68 kDa CAT, initiating at the second Met (residue No. 19), had no cleavable signal and was translocated into peroxisomes and cytosol, but not into mitochondria. The amino-terminal amino acid sequences of individually expressed CATs were identical to those of CATs isolated from alkane-grown C. tropicalis cells, respectively. These results demonstrated that only the 71 kDa protein yielded the 66 kDa protein and that peroxisomal and mitochondrial CATs arose from the difference in the initiation sites of translation.
- リンク情報
-
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902116199044796
- CiNii Articles
- http://ci.nii.ac.jp/naid/10005190224
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/8947834
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VL47100007&DestApp=WOS_CPL
- ID情報
-
- ISSN : 0021-924X
- J-Global ID : 200902116199044796
- CiNii Articles ID : 10005190224
- PubMed ID : 8947834
- Web of Science ID : WOS:A1996VL47100007