1998年10月
Hydrophobilization of esterase by genetic combination with polyproline or polytyrosine at the carboxyl terminal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
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- 巻
- 1388
- 号
- 1
- 開始ページ
- 239
- 終了ページ
- 246
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0167-4838(98)00170-8
- 出版者・発行元
- ELSEVIER SCIENCE BV
Polyproline (Poly-Pro) that is an amphiphilic polypeptide, or polytyrosine (Poly-Tyr) that is a hydrophobic polypeptide, were connected to the carboxy terminus of Pseudomonas sp. esterase by the recombinant DNA technique. The hydrophobicity of the esterase was enhanced by the introduction of Poly-Pro or Poly-Tyr, and also by increasing chain length of the polypeptides. Poly-Tyr increased the hydrophobicity of esterase more than Poly-Pro. Poly-Tyr induced significant conformational change of fusion esterase, but Poly-Pro did not. Consequently, the introduction of Poly-Tyr led to loss of activity of the fusion enzyme to a negligible level. On the other hand, the Poly-Pro-fusion-esterase retained enzymatic activity and the hydrolytic activity (k(cat)/K-m) of the fusion esterase carrying 40 proline residues (esterase-Pro40) relative to that of the wild-type esterase with the substrates p-nitrophenyl-propionate, -pentanoate, and -hexanoate was 1.76, 1.95, and 4.7, respectively. The results could be explained in terms of easier access of long-chain carboxylate to the fusion esterase compared to the wild-type esterase in aqueous solution. (C) 1998 Elsevier Science B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/S0167-4838(98)00170-8
- ISSN : 0167-4838
- PubMed ID : 9774739
- Web of Science ID : WOS:000076659800025