2001年5月
Genetically controlled self-aggregation of cell-surface-engineered yeast responding to glucose concentration
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- ,
- ,
- 巻
- 67
- 号
- 5
- 開始ページ
- 2083
- 終了ページ
- 2087
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/AEM.67.5.2083-2087.2001
- 出版者・発行元
- AMER SOC MICROBIOLOGY
We constructed an arming (cell-surface-engineered) yeast displaying two types of agglutinin (modified a-agglutinin and alpha -agglutinin) on the cell surface, with agglutination being independent of both mating type and pheromones. The modified a-agglutinin was artificially prepared by the fusion of the genes encoding Aga1p and Aga2p. The modified a-agglutinin could induce agglutination of cells displaying Ag alpha 1p (alpha -agglutinin). The upstream region of the isocitrate lyase gene of Candida tropicalis (UPR-ICL), active at a low glucose concentration, was used as the promoter to express the modified a-agglutinin- and alpha -agglutinin-encoding genes. The arming yeast displaying both agglutinins agglutinated and sedimented in response to decreased glucose concentration. When the glucose concentration was high, the arming yeast grew normally, In the late log phase, when the glucose concentration became very low, agglutination occurred suddenly and drastically and yeast cells sedimented completely. Sedimentation was confirmed by weighing the aggregated cells after filtration of the broth, Strains in which aggregation can be genetically controlled can be used in industrial processes in which the separation of yeast cells from the supernatant is necessary.
- リンク情報
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- DOI
- https://doi.org/10.1128/AEM.67.5.2083-2087.2001
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902154508112415
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11319085
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000168488400014&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1128/AEM.67.5.2083-2087.2001
- ISSN : 0099-2240
- eISSN : 1098-5336
- J-Global ID : 200902154508112415
- PubMed ID : 11319085
- Web of Science ID : WOS:000168488400014