2001年5月
Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- 巻
- 55
- 号
- 4
- 開始ページ
- 471
- 終了ページ
- 475
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s002530000539
- 出版者・発行元
- SPRINGER-VERLAG
The number of foreign protein molecules expressed on the cell surface of the budding yeast Saccharomyces cerevisiae by cell surface engineering was quantitatively evaluated using enhanced green fluorescent protein (EGFP). The emission from EGFP on the cell surface was affected by changes in pH. The amount of EGFP on the cell surface, displayed as alpha -agglutinin-fusion protein under control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, was determined at the optimum pH of 7.0. The fluorometric analysis and the image analysis by confocal laser scanning microscopy (CLSM) showed a similar number of molecules displayed on the cell surface, demonstrating that 10(4)-10(5) molecules of alpha -agglutinin-fused molecules per cell were expressed. Furthermore, the amount of fluorescent protein expressed on cells harboring a multicopy plasmid was three to four times higher than that on cells harboring the gene integrated into the genome.
- リンク情報
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- DOI
- https://doi.org/10.1007/s002530000539
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902176129715092
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11398929
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000168842900014&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/s002530000539
- ISSN : 0175-7598
- J-Global ID : 200902176129715092
- PubMed ID : 11398929
- Web of Science ID : WOS:000168842900014