2006年5月
Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- 巻
- 70
- 号
- 5
- 開始ページ
- 573
- 終了ページ
- 579
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00253-005-0101-z
- 出版者・発行元
- SPRINGER
Four types of cell-surface-engineered yeast Saccharomyces cerevisiae displaying glucoamylase, namely, systems A, B, C, and D, were constructed to evaluate their performance in direct ethanol fermentation from raw corn starch. Systems A and B were glucoamylase-displaying nonflocculent yeast (YF237) types that secrete alpha-amylase into the culture medium and codisplay alpha-amylase on the cell surface, respectively. Systems C and D were flocculent yeast counterparts (YF207) for systems A and B, respectively. In batch fermentations, the specific ethanol production rates of systems A, B, C, and D were 0.18, 0.06, 0.06, and 0.04 g (g cell)(-1) h(-1), respectively. In repeated fermentations, the specific ethanol production rate of system A decreased with the number of repetitions, whereas, that of system B was maintained. In all systems, the rate-limiting step was the conversion of starch to oligosaccharide because oligosaccharide and glucose were not accumulated throughout the fermentations.
- リンク情報
- ID情報
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- DOI : 10.1007/s00253-005-0101-z
- ISSN : 0175-7598
- J-Global ID : 200902218589147089
- Web of Science ID : WOS:000236624500009