2009年11月
Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 108
- 号
- 5
- 開始ページ
- 359
- 終了ページ
- 364
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2009.05.002
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
By application of the high-efficiency loss of heterozygosity (HELOH) method for disrupting genes in diploid sake yeast (Kotaka et al., Appl. Microbiol. Biotechnol., 82, 387-395 (2009)), we constructed, from a beterozygous integrant, a homozygous diploid that overexpresses the alcohol acetyltransferase gene ATF2 from the SEDI promoter, without the need for sporulation and mating. Under the conditions of sake brewing, the homozygous integrant produced 1.4 times more isoamyl acetate than the parental, heterozygous strain. Furthermore, the homozygous integrant was more genetically stable than the heterozygous recombinant. Thus, the HELOH method can produce homozygous, recombinant sake yeast that is ready to be grown on an industrial scale using the well-established procedures of sake brewing. The HELOH method, therefore, facilitates genetic modification of this rarely sporulating diploid yeast strain while maintaining those characteristics required for industrial applications. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.jbiosc.2009.05.002
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902279845496877
- CiNii Articles
- http://ci.nii.ac.jp/naid/110007481766
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000273247700001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.jbiosc.2009.05.002
- ISSN : 1389-1723
- J-Global ID : 200902279845496877
- CiNii Articles ID : 110007481766
- Web of Science ID : WOS:000273247700001