論文

査読有り
2010年4月

Microencapsulation of Islets with Living Cells Using PolyDNA-PEG-Lipid Conjugate

BIOCONJUGATE CHEMISTRY
  • Yuji Teramura
  • ,
  • Luan Nguyen Minh
  • ,
  • Takuo Kawamoto
  • ,
  • Hiroo Iwata

21
4
開始ページ
792
終了ページ
796
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/bc900494x
出版者・発行元
AMER CHEMICAL SOC

Microencapsulation of islets with a semipermeable membrane, i.e., bioartificial pancreas, is a promising way to transplant islets without the need for immunosuppressive therapy for insulin-dependent diabetes mellitus (type I diabetes). However, materials composing a bioartificial pancreas are not ideal and might activate defense reactions against foreign materials. In this study, we propose an original method for microencapsulation of islets with living cells using an amphiphilic poly(ethylene glyocol)-conjugated phospholipid derivative (PEG-lipid) and DNA hybridization. PolyA and polyT were introduced onto the surfaces of the islets and HEK 293 cells, respectively, using amphiphilic PEG-lipid derivatives. PolyA20 modified HEK cells were immobilized onto the islet surface where polyT20-PEG-lipid was incorporated. The cells spread and proliferated on the islet surface, and the islet surface was completely encapsulated with a cell layer after culture. The encapsulated islets retained the ability to control insulin release in response to glucose concentration changes.

Web of Science ® 被引用回数 : 40

リンク情報
DOI
https://doi.org/10.1021/bc900494x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20210336
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276817800028&DestApp=WOS_CPL

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