2008年9月
Suppression subtractive hybridization and differential screening reveals endodormancy-associated expression of an SVP/AGL24-type MADS-box gene in lateral vegetative buds of japanese apricot
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
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- 巻
- 133
- 号
- 5
- 開始ページ
- 708
- 終了ページ
- 716
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.21273/JASHS.133.5.708
- 出版者・発行元
- AMER SOC HORTICULTURAL SCIENCE
To understand the molecular basis of the endodormancy of buds of perennial plants, we searched for the genes that are expressed preferentially in endodormant lateral buds of the deciduous fruit tree japanese apricot (Prunus mume Sieb. et Zucc.) using suppression subtractive hybridization with mirror orientation selection (SSH/MOS). We generated two SSH/MOS libraries containing gene pools that arc! expressed preferentially in endodormant buds in comparison with paradormant or ecodormant buds to search for the genes that are upregulated by endodormancy induction or down-regulated by endodormancy release, respectively. Differential screening and sequencing indicated that genes involved in gibberellin metabolism, stress resistance, cell wall modification, and signal transduction, such as transcription factors, are upregulated in endodormant buds. After a further expression survey and full-length cDNA cloning, we found that a gene similar to the SVP/AGL24-type MADS-box transcription factor showed endodormancy-associated expression. Seasonal expression analysis suggested that the SVP/AGL24 homolog in japanese apricot might be involved in endodormancy regulation of its lateral buds.
- リンク情報
- ID情報
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- DOI : 10.21273/JASHS.133.5.708
- ISSN : 0003-1062
- eISSN : 2327-9788
- J-Global ID : 200902295190685057
- Web of Science ID : WOS:000259690100011