論文

査読有り
2004年4月

alpha 1-syntrophin modulates turnover of ABCA1

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Y Munehira
  • T Ohnishi
  • S Kawamoto
  • A Furuya
  • K Shitara
  • M Imamura
  • T Yokota
  • S Takeda
  • T Amachi
  • M Matsuo
  • N Kioka
  • K Ueda
  • 全て表示

279
15
開始ページ
15091
終了ページ
15095
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M313436200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

ABCA1 (ATP-binding cassette transporter A1) mediates the release of cellular cholesterol and phospholipid to form high density lipoprotein. Functions of ABCA1 are highly regulated at the transcriptional and post-transcriptional levels, and the synthesized ABCA1 protein turns over rapidly with a half-life of 1 - 2 h. To examine whether the functions of ABCA1 are modulated by associated proteins, a yeast two-hybrid library was screened with the C-terminal 120 amino acids of ABCA1. Two PDZ (PSD95-Discs large-ZO1) proteins, alpha1-syntrophin and Lin7, were found to interact with ABCA1. Immunoprecipitation revealed that alpha1-syntrophin interacted with ABCA1 strongly and that the interaction was via the C-terminal three amino acids SYV of ABCA1. Co-expression of alpha1-syntrophin in human embryonic kidney 293 cells retarded degradation of ABCA1 and made the half-life of ABCA1 five times longer than in the cells not expressing alpha1-syntrophin. This effect is not common among PDZ-containing proteins interacting with ABCA1, because Lin7, which was also found to interact with the C terminus region of ABCA1, did not have a significant effect on the half-life of ABCA1. Co-expression of alpha1-syntrophin significantly increased the apoA-I-mediated release of cholesterol. ABCA1 was co-immunoprecipitated with alpha1-syntrophin from mouse brain. These results suggest that alpha1-syntrophin is involved in intracellular signaling, which determines the stability of ABCA1 and modulates cellular cholesterol release.

リンク情報
DOI
https://doi.org/10.1074/jbc.M313436200
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14722086
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220594700074&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M313436200
  • ISSN : 0021-9258
  • PubMed ID : 14722086
  • Web of Science ID : WOS:000220594700074

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