2008年1月
Direct interaction of Cucurbitacin E isolated from Alsomitra macrocarpa to actin filament
CYTOTECHNOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 56
- 号
- 1
- 開始ページ
- 33
- 終了ページ
- 39
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s10616-007-9100-5
- 出版者・発行元
- SPRINGER
A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.
- リンク情報
- ID情報
-
- DOI : 10.1007/s10616-007-9100-5
- ISSN : 0920-9069
- PubMed ID : 19002839
- Web of Science ID : WOS:000251874000005