2005年
Measurement of receptor-binding activity of non-steroidal ecdysone agonists using in vitro expressed receptor proteins (EcR/USP complex) of Chilo suppressalis and Drosophila melanogaster
NEW DISCOVERIES IN AGROCHEMICALS
- ,
- ,
- ,
- 巻
- 892
- 号
- 開始ページ
- 191
- 終了ページ
- 200
- 記述言語
- 英語
- 掲載種別
- 書評論文,書評,文献紹介等
- 出版者・発行元
- AMER CHEMICAL SOC
N-tert-Butyl-N,N'-dibenzoylhydrazine and its analogs are agonists of insect molting hormone (20-hydroxyecdysone: 20E). In order to elucidate the mode of action of these nonsteroidal ecdysone agonists at the molecular level, we expressed the receptor protein for 20E - the heterodimer of ecdysone receptor (EcR) and ultraspiracle (USP) - in vitro, and analyzed their binding affinities to ligands. For the lepidopteran Chilo suppressalis, we showed quantitatively that the receptor-binding activity of non-steroidal ecdysone agonists determines the strength of their molting hormonal and insecticidal activities. The binding activity of five representative non-steroidal ecdysone agonists to C. suppressalis EcR/USP was higher than that to dipteran Drosophila melanogaster ECR/USP, which probably results in the selective toxicity between these two insects.
- リンク情報
- ID情報
-
- ISSN : 0097-6156
- J-Global ID : 200902254131100462
- Web of Science ID : WOS:000228806500018