2010年2月
A Novel Amphipathic Linear Peptide with Both Insect Toxicity and Antimicrobial Activity from the Venom of the Scorpion Isometrus maculatus
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- ,
- ,
- ,
- 巻
- 74
- 号
- 2
- 開始ページ
- 364
- 終了ページ
- 369
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.90723
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Scorpion venoms are composed of a number of peptides, many of which show neurotoxicity. In addition to these neurotoxins, several antimicrobial peptides have also been isolated from the venoms. The scorpion Isometrus maculatus, belonging to the Buthidae family, is found in many tropical regions including Japan, hut little attention has been paid to its biological activity and chemical composition. In this study, we isolated a novel insect toxin, Im-1, by bioassay-guided fractionation of the venom of L maculatus. Rapid and reversible paralysis was observed after injection of Im-1 into crickets. Im-1 consists of 56 amino acids, and is predicted to form an amphipathic alpha-helix. Since Im-1 shares sequence similarity to an antimicrobial peptide, parabutoporin, we evaluated its effects on several bacterial strains and found that it showed an antimicrobial activity profile similar to parabutoporin. This suggests that Im-1 and parabutoporin exert their antimicrobial effects through similar mechanisms.
- リンク情報
-
- DOI
- https://doi.org/10.1271/bbb.90723
- CiNii Articles
- http://ci.nii.ac.jp/naid/10027551635
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20139620
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000275175700021&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1271/bbb.90723
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10027551635
- PubMed ID : 20139620
- Web of Science ID : WOS:000275175700021