1998年
Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV
Microbiology and Immunology
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- ,
- ,
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- 巻
- 42
- 号
- 4
- 開始ページ
- 329
- 終了ページ
- 334
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1348-0421.1998.tb02291.x
- 出版者・発行元
- Blackwell Publishing Asia
Studies have indicated that cauliflower mosaic virus (CaMV) gene expression is mediated by the translation of polycistronic 35S pregenomic RNA, but the involvement of some minor subgenomic RNA species is also suspected. We examined the involvement of the 35S promoter in the expression of CaMV open reading frames (ORFs) I and IV using both 35S RNA-driven and promoter-less ORF I- and ORF IV-β-glucuronidase (GUS) fusion constructs. In addition to the 35S promoter-dependent expression of both ORF I- and IV-GUS fusions, we detected the 35S promoter-independent expression of both fusion genes via subgenomic mRNAs, which were detected by Northern blotting in the protoplasts transfected with the 35S promoter-driven constructs as well as in those transfected with the promoter-less constructs. These results suggest the involvement of subgenomic RNAs in the expression of CaMV ORFs I and IV, and the operation of a dual strategy in the expression of two viral genes.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1348-0421.1998.tb02291.x
- ISSN : 0385-5600
- J-Global ID : 200902196254584618
- CiNii Articles ID : 10005870878
- PubMed ID : 9623922
- SCOPUS ID : 0031958504