2008年12月
Synthesis of glyceryl ferulate by immobilized ferulic acid esterase
BIOTECHNOLOGY LETTERS
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- 巻
- 30
- 号
- 12
- 開始ページ
- 2151
- 終了ページ
- 2156
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s10529-008-9814-2
- 出版者・発行元
- SPRINGER
Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50 degrees C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25 degrees C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.
- リンク情報
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- DOI
- https://doi.org/10.1007/s10529-008-9814-2
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18688576
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000260306800018&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=54849417360&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=54849417360&origin=inward
- ID情報
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- DOI : 10.1007/s10529-008-9814-2
- ISSN : 0141-5492
- eISSN : 1573-6776
- PubMed ID : 18688576
- SCOPUS ID : 54849417360
- Web of Science ID : WOS:000260306800018