2013年
Functional analysis of lysosomes during mouse preimplantation embryo development
Journal of Reproduction and Development
- 巻
- 59
- 号
- 1
- 開始ページ
- 33
- 終了ページ
- 39
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1262/jrd.2012-096
Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our fndings highlight the functional changes in lysosomes in mouse preimplantation embryos. © 2013 by the Society for Reproduction and Development.
- ID情報
-
- DOI : 10.1262/jrd.2012-096
- ISSN : 0916-8818
- ISSN : 1348-4400
- PubMed ID : 23080372
- SCOPUS ID : 84874343163