2007年9月
Metabolomics for metabolically manipulated plants: effects of tryptophan overproduction
METABOLOMICS
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- ,
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- 巻
- 3
- 号
- 3
- 開始ページ
- 319
- 終了ページ
- 334
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s11306-007-0072-4
- 出版者・発行元
- SPRINGER
Advances in molecular breeding technologies have enabled manipulation of the concentrations of specific plant components by modifying the genes that play a key role in their production. This has provided new opportunities to enhance the nutritional quality of major crops. However, given that metabolic pathways form a highly integrated network, any alteration in a given biosynthetic pathway is most likely to effect secondary and unpredicted changes in the metabolite profile of other pathways. Metabolomics technologies can contribute to the efficient detection of such unexpected effects caused by genetic modification. This has relevance not only from the perspective of safety evaluations of newly developed crops, but to basic science focused on uncovering hitherto unknown regulatory mechanisms associated with the biosynthesis and catabolism of primary and secondary metabolites in plants. In this review, recent advances in plant metabolic engineering for the overproduction of tryptophan (Trp), one of the essential amino acids, are described. In particular, the efficacy of a transgene OASAID that encodes a mutant anthranilate synthase (AS) alpha subunit of rice in specifically elevating levels of Trp without marked secondary effects on the metabolite profile of rice is demonstrated. Related topics, such as regulation of Trp biosynthesis, possible interactions between the biosyntheses of Trp and other aromatic amino acids, and translocation of Trp in are discussed based on findings derived from metabolomic analyses of Trp-overproducing transgenic plants.
- リンク情報
- ID情報
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- DOI : 10.1007/s11306-007-0072-4
- ISSN : 1573-3882
- eISSN : 1573-3890
- Web of Science ID : WOS:000249945500017